[Vor. 3 
284 ANNALS OF THE Missourt BOTANICAL GARDEN 
rapidly liquefied. Saccharose, dextrose, levulose, and mal- 
tose are fermented. 
Culture 13, Saccharomyces cerevisiae—This yeast, ob- 
tained from “Yeast Foam,’’ has spherical cells (fig. 13) vary- 
ing in size from 5 to Әріп diameter. Growth takes place by bud- 
ding from all sides of the mother cell. In nutrient sugar solu- 
tions the organism forms a sediment of yeast cells and a 
thin film only in the presence of glucose. In yeast water 
containing organic acids, the sediment of yeast cells has a 
floceulent appearance. Saccharose, dextrose, levulose, and 
Fig. 12. Culture 12. Vegetative cells, conidia, chlamydospores, and muri- 
form many-celled spores. 
maltose are fermented, with the formation of aleoliol and 
carbon dioxide. Spores are formed on moist porous plates, 
on Gorodkowa’s test medium, and on yeast-water agar. On 
the latter medium, after a growth of ten days, 30-40 per cent 
of the yeast cells have formed endospores. The number of 
ascospores in the asci varies from 1 to 4, and they vary 
in size from 2.5 to 4и in diameter. Upon germination the 
spores enlarge and are set free from the spore case and 
then develop separately, or the spores may fuse as they 
become larger, the spore case becoming thinner at the same 
time, which results in a very large cell that develops by 
budding. Sometimes the ascospores start to germinate be- 
fore they are set free from the ascus. 
The reaction of the thirteen organisms was tested in 
nutrient solutions of saecharose, dextrose, levulose, and mal- 
