1916] 
Ewic—Yzasr F'UNGI 285 
tose. The sugar nutrient solutions contained 1 per cent 
peptone, .3 per cent monopotassium phosphate, and .02 per 
cent magnesium sulphate. The concentration of the sugar 
was 5 per cent saccharose, D per cent dextrose, 1 per cent 
levulose, and 1 per cent maltose, respectively. 
For the determination of alcohol formation, 100 ce. of the 
sugar nutrient solutions were placed іп 125-ce, Erlenmeyer 
^ COQ 
Fig. 13. Culture 13. Vegetative cells, asei with ascospores, and spores dur- 
ing germination. 
flasks, and after sterilization each was inoculated with a pure 
culture of one of the thirteen different strains and incubated 
at room temperature for thirty days. Immediately after 
the inoculation of the nutrient solutions the cotton plugs 
in the culture flasks were replaced by one-holed rubber 
stoppers, through which passed a piece of small glass tubing 
with a small cotton plug. This precaution was taken to 
prevent excessive evaporation. After the incubation at 
room temperature for thirty days, the nutrient solutions were 
neutralized with normal caustic soda and made up to the 
