[Vor. 3 
286 ANNALS OF THE Missourt BOTANICAL GARDEN 
original volume with distilled water. The loss by evapora- 
tion did not exceed .3 ce. in any case. Fifty се. were then 
distilled off, and the amount of alcohol present determined 
with a pyenometer. The presence of aleohol was confirmed 
in each case by means of the iodoform test as used by Will 
(710) in his investigation on certain species of Mycoderma. 
TABLE I 
SHOWING THE PERCENTAGE, BY VOLUME, OF ALCOHOL PRODUCED BY THE 
THIRTEEN DIFFERENT CULTURES AFTER INCUBATION AT 
OOM TEMPERATURE FOR THIRTY DAYS 
Iodo- Iodo- Iodo- Iodo- 
үе SE form ман form | — | form | Maltose | form 
| test test test test 
1 .10 —* .10 — „10 -- 30 -— 
2 05 — ‚05 — .10 — 20 -— 
8 .00 — .10 -- 10 -- 00 -- 
4 15 -- .00 - 10 — .20 -- 
5 00 -- 00 ЕЕ 10 05 -— 
6 .00 -— .85 + 80 + .10 -— 
7 .95 4 .65 -- 15 -- 25 - 
8 1.20 -р 45 + .10 — 45 + 
9 00 — 00 - 05 -- .20 — 
10 .10 — .10 -- .05 -— .00 -— 
11 .95 + ‚85 -- 115 -- ‚55 + 
12 80 + 1.00 + .50 + „75 + 
13 5.35 + 5.00 + „70 + .85 + 
Control .00 -- 00 -- 00 -- 00 — 
(without 
TE 
ism) 
*The sign + indicates that the iodoform test for aleohol was positive, with 
the presence of iodoform erystals. 
The results, as indicated by table r, show that very little 
aleohol, if any, is formed by the various eultures with the 
exception of culture 13 (Saccharomyces cerevisiae), whieh 
was used as a control Only one culture of Torula, culture 
6, produced alcohol by the fermentation of dextrose and 
levulose, Four of the black yeast-like fungi-cultures, 7, 8, 11, 
and 12, produeed only small quantities of alcohol. 
The reaction of the thirteen different cultures was tested 
in yeast water containing organic acids. Seventy-five grams 
of press-yeast were ground up with enough distilled water 
to form a thin paste. More distilled water was then added 
