[Vor. 3 
290 ANNALS OF THE Missourt BOTANICAL GARDEN 
TABLE III 
SHOWING THE CHANGE IN ACID REACTION E ABOUT BY THE THIR- 
TEEN gie = DURING THE INCUBATION AT ROOM 
RATURE FOR THIRTY DAYS 
(Results are the averages of the two titrations given in table IT) 
Culture no. Acetic || Citrie | Lactic Malie Sueeinie | Tartarie 
1 + .15* --6.0 --1.5 —2.65 —3.6 — .95 
2 + .45 --6.1 --4.3 —5.8 —6.3 —2.2 
3 A —2.4 —1.1 —5.35 —6.15 — 9 
4 —6.15 —6.05 1 —5. —6.2 + 1 
5 + A --5.6 --1.8 —5.8 --6.15 — 4 
6 + .25 —6.2 —3.7 —5.9 —6.1 — 6 
T + .55 —6.1 —2.85 D —6.5 —5.65 
8 +1.3 --6,15 —2.15 —5.95 —6.5 --5.65 
9 + D + .3 + .2 + .15 — 35 1.5 
10 +1.85 —1.2 --1.4 --9. --6.15 0.0 
al + 6 —6.3 --9.15 --5.9 --6.5 --5.65 
12 +1.25 —2.7 --1.35 --5.6 —6.3 — .7 
13 + .25 +1.0 | --2.1 -1.75 —2.2 +3.5 
*In terms of N/10 caustic soda per 10 ce. culture solution. 
The results, as indicated by tables rr and пт, show that 
all the eultures produce a change in the reaction of the acid 
yeast-water solutions after ineubation at room temperature 
for thirty days. There is a decrease in the acidity of all 
the acid nutrient solutions except in the presence of acetic 
acid, in which ease a marked decrease in acidity was brought 
about only by culture 4. In the case of culture 9, only a 
slight change of acidity was found in any of the acid nutrient 
solutions. 
ANIMAL EXPERIMENTS 
The experiments on animals were made in the laboratory 
of the pathological department of the Washington University 
Medical School, under the direction of Dr. E. L. Opie and 
Dr. W. S. Thomas, Rabbits and guinea-pigs were inoculated 
with suspensions of the different yeast-like fungi, with the 
exception of cultures 3 and 13, either from cultures grown 
in Hansen’s solution for 12 hours, or from 48-hour cul- 
tures grown on agar and suspended in Ringer’s physiologi- 
cal salt solution. In each case 2 ce. were injected into the 
'Ringer's solution contains the followi 
ing: 
Sodium chloride...... .7 per cent Calcium chloride...... .028 per cent 
Potassium diei. ... 035 per cent Sodium carbonate. .... .003 per cent 
