1916] 
Emic—Yerast FUNGI 291 
marginal ear vein of rabbits, or 1 ce. intraperitoneally or 
subcutaneously in the guinea-pigs. All injections were made 
under aseptic conditions with sterilized instruments. 
The animals that died were carefully examined for lesions 
or abnormalities, the autopsy being carried out with aseptic 
precautions with sterilized instruments. At the same time 
a sample of blood was taken from the right auricle of the 
heart, with a sterilized platinum loop, and transferred to a 
tube containing sterilized beer-wort. All the principal organs 
were removed to sterilized Petri dishes, and then small 
pieces of the liver, lungs, spleen, kidneys, and intestines were 
placed in tubes of sterilized beer-wort. These tubes were 
then incubated at 28°C. for seventy-two hours, but were 
kept under observation for over ten days, and then gelatin 
plates made from them. 
Where the organisms had been inoculated into beer-wort 
and the same procedure used as for the animal tissues, pure 
cultures were again easily obtained on gelatin plates with 
all the eleven strains used. 
At the same time pieces of the different organs were 
fixed in Bouin’s or Gilson’s fluid, imbedded in paraffin, 
sectioned, and stained with saffranin and methyl blue, or 
with Delafield’s hematoxylin and eosin, or according to the 
method used by Pianeze (796) for the staining of carcinoma 
tissue. 
