[Vor. 3 
294 ANNALS OF THE Missovrt BOTANICAL GARDEN 
preparations revealed a condensation of the air spaces of 
the lungs. There were no organisms present and this con- 
dition was probably due to post-mortem changes such as 
oedema. 
In experiment 3 animal No. 7 died after seven days, and 
the autopsy revealed the intestines very much inflamed. 
The organism was isolated from the intestines, and stained 
preparations showed abnormalities in the condensation of 
air spaces in the lungs. А repetition of this experiment 
gave negative results. 
In experiment 4 animal No. 11 died in less than two days 
from causes that could not be determined. The death of 
this animal may have been due to the fact that phagocytosis 
was not sufficiently active, since a repetition of the same ex- 
periment gave negative results. 
In experiment 5 the results were negative. 
In experiment 6 animal No. 19 died after twenty days 
without evidence of lesions. To test out the production of 
toxin by culture No. 7, 1 ce. of a four-day culture in beer- 
wort after sterilization was injected intravenously into a 
660-gram rabbit, but the results were negative. 
In experiment 7 animal No. 21 died after twenty days 
from unknown eauses. The condensation of air spaces 
found in the lungs was probably due to post-mortem changes. 
Animal No. 22, а 780-gram rabbit, was subjected to a 1-ce. 
sterilized injeetion of a four-day eulture of organism No. 8, 
with negative results. 
In experiment 8 animal No. 23 died after two months with- 
out evidence of lesions other than white spots on the liver 
which were presumably due to coccidia, since no fungous cul- 
ture was obtained from this lesion. 
In experiment 9 animal No. 26 died after ten days from 
unknown causes. Intravenous injection in an 870-gram 
rabbit of a l-ee. four-day sterilized culture grown in beer- 
wort, gave negative results. 
In experiment 10 animal No. 27 died after twenty days 
without evidence of lesions or the presence of fungi in the 
various organs. 
