[Vor. 8 
302 ANNALS OF THE Missourt BOTANICAL GARDEN 
except in the presence of acetic acid and in culture No. 4. 
The results of thirty-four animal experiments were neg- 
ative, in that the death of certain animals was not caused 
by the formation of lesions or abnormalities due to the 
organisms injected. 
No extracellular toxins were obtained from the cultures 
of these twelve yeast-like organisms. 
The results of these experiments and a review of literature 
on animal pathology indicate either that pathogenic yeast- 
like fungi do not occur in nature, or that if they are present, 
they are so few as to be met with only under exceptional 
conditions, 
In conclusion, the writer wishes to express his thanks to 
Professor George T. Moore, for the suggestion of the prob- 
lem and for numerous courtesies extended during the prog- 
ress of the investigation while at the Missouri Botanical 
Garden; to Dr. E. L. Opie and Dr. W. S. Thomas, for sug- 
gestions and aid in the animal experiments which were 
carried out at the Washington University Medical School; 
Professor W. C. Allee, for various suggestions and assistance 
in certain of the animal experiments; to Dr. Gayfree Ellison, 
for courtesies extended at the Bacteriological Laboratory of 
the University of Oklahoma; and to Mr. G. H. Smith of 
the University of Oklahoma, for assistance in reading the 
proofs. 
Graduate Laboratory, Missouri Botanical Garden. 
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