[Vor. 3, 1916] 
410 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
showing some of the steps involved in the enzymie hydrolysis 
of albumin. 
Papain (.1 gm.) was added to 200 ee 2.5 per cent albu- 
min solution, alkalinity reduced to N/250, then incubated at 
40°C. for two hours. Portions were removed and tested both 
with the ‘‘micro’’- and the ‘‘macro’’-Kjeldahl for proteolytic 
change. Опе се. was digested with the former, 15 се. were 
used with the latter. 
NITROGEN DETERMINATIONS IN THE HYDROLYSIS OF ALBUMIN 
Micro-Kjeldahl | Macro-Kjeldahl 
Nitrogen Albumin | Albumin |Albumin |Albumin |Albumin |Albumin 
fractions +епғуте | +water |--епгуте| -+water |-+enzyme| +water 
in 1 cc. calc. to 15 cc. in 15 cc. 
mg. mg. mg. mg. mg. mg. 
Total nitrogen.... 3.80 3.795 57.00 56.925 57.615 57.500 
oagulable 
nitrogen........ .913 3.145 13.695 | 47.175 14.125 48.225 
Phosphotungstate 
РЧ р 1.397 .472 20.955 7.080 21.275 6.925 
Ee acids and 
МА ree ree 1.490 177 22.35 2.655 22.5025 2.785 
Further comparison is made in the recovery of nitrogen 
from a carefully prepared solution of (NH4)»SO,. As be- 
fore, 1 ec. of solution was used with the ‘‘micro’’- and 15 се. 
with the ‘‘macro’’-Kjeldahl. 
TABLE II 
THE RECOVERY OF MEE ter A SOLUTION OF (NH4:S50. CONTAINING 
217 MGS. PER CC. 
Micro-Kjeldahl, Macro-Kjeldahl 
Exp. 1 cc. solution used 15 cc. solution used 
no. 
Found Calculated for 15 cc. Found 
mg. mg. mg. 
1 3.13 46.95 47.595 
2 3.12 46.80 48.125 
3 3.17 47.55 46.925 
4 3.185 47.775 45 .400 
5 3.192 47.880 47.3925 
Theoretical 3.217 48.255 48.255 
Graduate Laboratory, Missouri Botanical Garden. 
