[Vor. 3 
422 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
sults: Alternaria 1.02-5.55 mg., Hormodendron .36-5.0 mg., 
Macrosporium .23-5.91 mg. 
Experiments conducted by Kossowiez ('12) which seemed 
to suggest N-fixation in the case of certain species of Sac- 
charomyces, Monilia candida, and Oidium lactis were subse- 
quently repeated by him (714) under more nearly standard 
control conditions. The results were interpreted as entirely 
negative. Besides the organisms previously employed, he 
used also Aspergillus niger, A. glaucus, Penicillium glaucum, 
P. brevicaule, and a species of each of the following, Botrytis, 
Mucor, and Isaria. 
Will (212), reporting work of Scheckenbach, declared the 
capacity of certain species of Torula to grow upon nutrient 
media lacking nitrogen, likewise to fix atmospherie nitrogen 
when little or no combined nitrogen was supplied. "There is, 
however, with the experiments reported, little evidence that 
sufficient precautions were taken in the arrangement of suit- 
able controls. 
The capacity of Blastoderma salmimcolor, Torula sp., and 
**pastorianus"" yeast to fix nitrogen has been mentioned by 
Lindner (712),! but to what extent this work was quantita- 
tively executed cannot be determined from the data at hand. 
Goddard's (713) investigations parallel those of Froehlich, 
Stahel, and, to a certain extent, those of Ternetz. He isolated 
15 species of fungi from the soil, and tested each of these with 
respect to nitrogen fixation, grown on 50 cc. of a culture solu- 
tion eomparable to the nutrient media employed by other in- 
vestigators. Every possible precaution seems to have been 
taken to insure accuracy. The fungi were grown 48-70 days. 
With no organism in any series were there indications of con- 
sistent gains over the initial nitrogen content. Species of 
Aspergillus and Penicillium were included in these studies. 
In connection with his investigations of mycorhiza problems, 
Peklo (713) isolated 3 species of fungi, 2 being species of 
Penicillium, and one an indetermined form. Each of these 
was grown on Winogradski's solution plus dextrose for 1-2 
1 The reference given appears to be an abstract of a more extensive report 
which is at present unavailable. 
