[VoL. 3 
440 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
anastomosed that the pores formed are very much daedaloid. 
The mycelium is best studied in pure cultures. 
CULTURE RELATIONS 
Two methods of securing pure cultures were employed. 
They may be designated as (1) the tissue method, and (2) the 
spore method. 
(1) The tissue method was first described by Duggar (’05), 
who applied this method to the making of spawn in mush- 
room culture. In connection with this study on Agaricus 
campestris tests were made with 69 species of basidiomy- 
cetous fungi on various media. Forty of these grew promptly 
on the media employed. The method was suggested by the 
fact that ‘‘during moist weather, or in a moist cellar where 
mushrooms are being grown, one will frequently find that an 
injury in a young mushroom is rapidly healed by a growth 
of hyphae from the edges of the injured area. The same thing 
had been noticed in the open in the case of puffballs. In 
many instances, moreover, pure cultures of fungi in other 
groups have been obtained by small bits of a sclerotial mass 
of tissue.” Accordingly, young sporophores were obtained, 
“апа after breaking them open longitudinally a number of 
pieces of tissue from within were carefully removed with a 
sterile scalpel to a sterile Petri dish." А number of cultures 
were then made by transferring these nocules of tissue to 
various forms of nutrient media, such as bean pods, manure, 
leaf mould, ete. From this and from numerous other similar 
tests it was ascertained that when the sporophores from 
which the nocules of tissue were taken were young and 
healthy, there was seldom an instance in which growth did 
not result. It was shown that failure to grow was generally 
due to the advanced age of the sporophore used, to an un- 
favorable medium, or to bacterial contamination. 
In my work dried sporophores were used for the tissue 
method. These were collected in a freshly growing condition 
and dried at room temperature. The sporophores of Lenzites 
saepiaria are xerophytie, and will remain viable in a dried con- 
dition for some time. Buller (209, p. 111) found them to 
