[VoL. 3 
460 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
enzyme. The sucrose solution was also used alone. These 
were all set up in duplicate, and at the same time parallel 
experiments were set up with sporophoral meal in the same 
way. They were allowed to remain at room temperature 
(about 27°C.). After a period of 3 hours one set of cultures 
was killed in the autoclave by allowing the pressure to come 
to 10 pounds. The duplicate set remained in incubation for 
TABLE V 
QUANTITATIVE STUDY ei THE INVERTASE ACTIVITY IN MYCELIUM 
D SPOROPHORES 
Reducing sugars as glucose Net invert sugar due to 
in 10 cc. of substrate invertase activity 
Amount of fungous | After 3 hrs. | After 6 hrs. | After 3 hrs. | After 6 hrs. 
powder in 50 сс. of 1 | incubation | incubation incubation | incubation 
per cent sucrose solu- 
n Ф Ф Ф Ф 
Е 8 Е E Е 8 Е E 
3181318/318] ЕК 
See ee HERR ai 
= A = 27 = A = N 
mg. | mg. | mg. | mg mg. | mg. | mg. | mg. 
A WOU. SS ca ee IT 7.52 | 2.88 |17.19| 5.16 | 5.44 | 2.25 115.05] 4.53 
2 grams (autoclaved)... .| 2.08 | 0.63 | 2.14] 0.63 |......]......]......]...... 
Sucrose solution alone. . .| Negli- Negli- 
gible |...... Са А TET METEO КЕСИК с Mrs 
6 hours and then was killed in the same way. After killing 
the substrates were filtered, and the invert sugars were de- 
termined by the Shaffer method. Table v gives these quanti- 
tative results. "These results show definitely that the greater 
invertase activity is in the vegetative part of the fungus, the 
inversion taking place over twice as rapidly in the mycelium 
as in the sporophores. In collecting such sessile sporophores 
as those of L. saepiaria it is diffieult at times to remove the 
fruiting body without taking with it some superficial myce- 
lium. It may be that if only the marginal portions of the 
sporophores were used in this work there would be a greater 
difference in the activity between fruiting body and mycelium. 
RAFFINASE 
A earbohydrase which transforms the trisaccharide raf- 
finose into fructose and melibiose was demonstrated. To 10 
