1916] 
ZELLER—PHYSIOLOGY OF LENZITES SAEPIARIA 465 
Parallel experiments were prepared using the ground sporo- 
phores. The results of these experiments are incorporated 
in table уп. 
As was the case with the invertase, the diastatic activity 
shows up much more strongly in the mycelium than in the 
sporophores. 
TABLE VII 
QUANTITATIVE STUDY OF THE DIASTATIC ACTIVITY IN MYCELIUM 
AND SPOROPHORES 
Reducing sugars as glucose Net due to hydrolysis 
in 10 cc. of substrate y diastase 
After 3 hrs. | After 6 hrs. | After 3 hrs. | After 6 hrs. 
Amount of fungous incubation | incubation | incubation | incubation 
powder in 50 cc. of 1 
per cent starch paste Ф Ф Ф Ф 
ELS [28 | 8 |a е s= 
Е & | Е a | Е & | 5 & 
Š 2 5 - Š S 8 р 
E E & ^ & T & 
= a = 27 = Ф = г 
mg. | mg mg mg. | mg mg. | mg. | mg. 
ams powder........ 10.67 | 3.18 | 24.68] 7.96 | 8.50 | 2.39 | 22.54] 7.20 
2 о» lebt зы (auto- 
e 17 79 | 2.14 (LEE EE, EE BEA 
Starch paste alone...... Negli- Negli- 
е Gs ео. E ER 
CYTO-HYDROLYZING ENZYMES 
Under the general term cyto-hydrolyzing enzymes, I shall 
consider all enzymes which attack such higher carbohydrates 
as lignin, cellulose, hemicellulose, and pectic bodies. In the 
succeeding pages the following classification of this group 
of enzymes will be used: 
1. Ligninase, called ‘‘hadromase’’ by Czapek (799), to 
designate the enzyme capable of splitting lignin. 
2. Cellulase, the true cellulose-hydrolyzing ferment. 
3. Hemicellulase, the ferment hydrolyzing the hemicellu- 
loses. 
4. Pectase, the enzyme capable of clotting the pectins. 
9. Pectinase, an enzyme which hydrolyzes into reducing 
sugars the pectinous substances, especially the middle lamellae 
of plant tissues. 
