[VoL. 3 
470 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
that the same enzyme preparation is capable of hydrolyzing 
the free cellulose of the inner lamellae and leaving only such 
substances as will give a yellow reaction with chloriodid of 
zinc; and that the active substance in the preparation is 
thermolabile. 
The aqueous solution, just as it was filtered from the shav- 
ings, reduced Fehling’s solution; that from the second and 
third tubes reduced Fehling’s somewhat, but in comparison 
not so strongly as that from the first. Thus, quantitative de- 
terminations were made to decide whether the reducing sub- 
stances were due to enzyme action or possibly to the presence 
of other reducing substances already in the wood, as, for in- 
stance, tannins. 
То this end 10 сс. of the aqueous solution from each were 
placed in Erlenmeyer flasks of 125 ee. capacity. A fourth 
determination was made as a control on the Fehling’s solu- 
tion. The determinations of reducing sugars were made as 
glucose by the Shaffer method (74). Table уш gives Ше 
results obtained: 
TABLE VIII 
REDUCING SUGARS DUE TO ENZYME ACTION OF LENZITES SAEPIARIA 
ON PINE SHAVINGS IN 15 DAYS 
Tube . Reducing Net 
number Experiment substances bove 
as glucose | controls 
mg. mg. 
1 1 gm. pine + Va cc. enzyme dispersion + 15 cc. 
distilled water.............................. 10.606 8.973 
2 1 gm. pine + 30 cc. distilled water.............. 1.492 | raps 
3 1 gm.pine 4- 15 cc. раста chu (autoclaved) 
am E. IE MM ROMPE D 1.698. | рай 
4 Fehling’s aps сесин e sins x TENERE T Negligible | ........ 
The results obtained here show that these reducing sub- 
stances are due to enzyme action, and that they must be 
sugars, probably glucose and other monosaccharides. The 
sources of this glucose may be various. There is probably 
some tannin, although slight, in sap-wood. Besides this there 
is the hydrolysis of cellulose to reducing sugars, as demon- 
strated by the experiments reported later on cellulase. Then 
