[Vor. 3 
474 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
sugars, which were probably utilized by the fungus as fast 
as they were split off. I 
Wolf ('16) made poured plates of cellulose agar which he 
inoculated with species of Pseudomonas, Phoma, Gloeo- 
sporium, and Fusarium. He says: “There was no evidence 
of the production of cellulase except by Phoma.’’ Samples of 
normal tissues and of tissues diseased by Phoma socia Wolf 
were tested for cellulose by employing Schweitzer’s reagent, 
and Wolf observes that ‘‘there is a slight but significant de- 
crease in the amount of cellulose found in diseased tissues."' 
A considerable number of determinations consistently showed 
that “the lesser amount of cellulose was invariably found in 
the diseased tissue.’’ 
Further experiments were instituted, since it was clear from 
microchemical tests and from the above experiments on lig- 
ninase that cellulose of attacked wood disappears. In order 
to test out the action of the enzyme preparations on normal 
cellulose, pure cellulose from two sources was prepared. 
One was prepared from filter paper in the way described by 
McBeth and Scales (713) and later by Cooley (714). Fifteen 
grams of filter paper were dissolved in Schweitzer’s reagent 
and precipitated with dilute hydrochloric acid. After wash- 
ing thoroughly with dilute acid to get rid of all of the copper 
and then with distilled water to get rid of all of the chlorine, 
a very flocculent cellulose precipitate was obtained. The 
water was filtered off with a Buchner funnel until the cellulose 
suspension was concentrated to about 500 cc. This was trans- 
ferred to a liter flask which was plugged and sterilized. 
Another type of pure cellulose was made from pine wood. 
A quantity of fine pine shavings were treated with a cold solu- 
tion composed of 30 grams of potassium chlorate dissolved in 
520 се. of nitric acid (sp. gr. 1.1). The container was kept cold 
for 4 weeks, after which time the cellulose was washed and 
then precipitated from Schweitzer’s reagent, as in the above 
case. It was sterilized and kept for future use, as was the 
filter paper cellulose. 
Several types of cellulose agar were prepared, using both 
the filter paper cellulose and the pine wood cellulose. Some 
