1916] 
ZELLER—TPHYSIOLOGY OF LENZITES SAEPIARIA 475 
standard nutrient solutions for the artificial culture of fungi 
were used as a basis for these, such as: 
(1) Richards’ (797) solution, substituting 100 ec. of the 
2 cellulose suspensions for cane sugar and adding 2 grams of 
agar. 
БЕС ec MO E 10 gram 
ОООО г... 05 gram 
E EE 0.25 gram 
Ss PR EE 0.002 gram 
Cellulose suspension..... 100 ec. 
E E EE 2 grams 
(2) Cooley’s solution “A,” just as given by Cooley (714, 
p. 306). 
(3) Reed's solution, as given by Reed (713, p. 69), with 
the exception of using one-half as much distilled water to- 
gether with 500 ec. of cellulose suspension and 2 per cent agar. 
On any of these agars L. saepiaria grows very slowly and 
without producing much of a mat. Wherever cellulose 
hydrolysis could be seen it was very, very slight, and this was 
only in cases where the pine wood cellulose was used. 
Sinee these experiments gave such meagre results 2 per 
cent agars were made, using dilute extracts of carrot, turnip, 
and potato as bases and using cellulose suspensions in ap- 
proximately the same concentrations as in the above. In this 
series of experiments the cloudiness of the agar due to cellu- 
lose suspension was cleared up noticeably in one case only. 
This was where carrot-pine-wood-cellulose agar was used. 
The agar was tubed and sterilized in test-tubes of 13 mm. 
diameter. The agar was not slanted, and after inoculation 
the tubes were kept in a damp chamber so that the water con- 
tent of the agar would remain the same throughout incuba- 
tion. These tubes were kept at a temperature of 32°C. for 
4 weeks. After this length of incubation the agar had cleared 
to an average depth of 9 mm. in the inoculated tubes where 
carrot-pine-wood-cellulose agar was used. The uninoculated 
tubes were still uniformly cloudy. There was no hydrolysis 
in the tubes where carrot juice was not used. The most sig- 
nificant fact brought out in this series of experiments is that 
