[VoL. 3 
476 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
the pine wood cellulose is hydrolyzed by the cellulase excreted 
by L. saepiaria, while the filter paper cellulose remained 
untouched. 
Experiments with the enzyme dispersion were set up accord- 
ing to the scheme as outlined in table 1x. All of the experi- 
ments were set up in duplicate with both sporophoral and 
mycelial dispersions. Toluol was used as an antiseptic, and 
qualitative results were taken after 4 weeks. 
TABLE IX 
d EXPERIMENTS SHOWING THE ACTION OF MYCELIAL AND SPO- 
ORAL ENZYME DISPERSIONS ON F pilo е CELLULOSE 
D PINE WOOD CELLULO 
Reduction of Fehling's solution 
Experiment 
Mycelium Sporophore 
10 cc. paper cellulose + 2 cc. dispersion. ......... + + + 
10 cc. pine lose + 2 dispersion............ + + + + + + 
10 cc. pine cellulose + 2 cc. dispersion (autoclaved) + — 
10 cc. paper cellulose +2 cc. dispersion EE d)| + -- 
10 сс. paper cellulose + 2 сс. distilled water........ = Sp 
10 cc. pine cellulose +- 2 cc. distilled cn? Kee, — - 
Since such definite results were obtained with the mycelial 
enzyme dispersion in the foregoing experiments, it was de- 
termined to make quantitative comparisons of the activity of 
cellulase in the sporophoral and mycelial tissues. To this end 
a carrot extract was made as described above under ‘‘inver- 
tase,’’ and to this extract was added pine wood cellulose. This 
nutrient solution was placed in Erlenmeyer flasks, which were 
plugged, sterilized, and inoculated as described for invertase 
and diastase. The mats of mycelium formed after 2 weeks 
were removed from the flasks, dried, and ground to a fine 
powder which was used in the following way: To 50 cc. of the 
pine-cellulose suspension were added 2 grams of the mycelial 
powder with toluol as an antiseptic. Some of the mycelial 
powder was autoclaved, and controls on the above were pre- 
pared with this autoclaved powder as well as with distilled 
water alone. These experiments were set up in duplicate. A 
parallel series was prepared using the sporophoral tissue 
powder. After incubating these enzyme cultures at about 
