1916] 
ZELLER—PHYSIOLOGY OF LENZITES SAEPIARIA 485 
(2) After soaking sections in iodine and then treating 
with 65 per cent sulphuric acid, the secondary lamella was a 
yellow to brown color, with a purple lining in the early spring 
wood. 
(3) Chloriodid of zine gave a brown coloration both in the 
spring and summer wood, with a slight blue tint lining the 
tracheids in the sap-wood. 
(4) Aniline sulphate produced a bright yellow which in- 
creased in intensity from spring to late summer wood. 
(5) After treating with potassium hydroxide for some 
time and then applying the cellulose tests the following results 
were obtained: 
(a) After continued action of iodine followed by the 
addition of sulphuric acid, the inner or tertiary lamella 
was swollen and shrunken away from the secondary, the 
former assuming a purple color. 
(b) Chloriodid of zine colored the swollen tertiary 
lamella blue, while the main part of the wall was brown. 
(6) Resorcin and sulphuric acid gave a violet to blue re- 
action in the lignified walls. 
When these same tests were applied to the decayed wood 
some difficulty was found, especially where the tests yield 
yellow or brown, because the tissues were decidedly brown in 
the last stages of decay. If a transverse section is made 
through a decayed portion of wood so as to include a part of 
the sound, normal wood, the progressive stages of decay may 
be followed by applying the above stains. 
When phloroglucin and hydrochlorie acid are applied to 
such a section, it is noticed that chemical changes have pre- 
ceded any visible or microscopical changes in structure. In 
the sound wood the secondary and tertiary lamellae are 
stained a dark red, while the middle lamella is a still darker 
red. A little nearer the edge of the decayed region the red 
has changed to a maroon or brownish red in the tertiary 
lamella. As we proceed nearer to the decayed portion this 
maroon increases until the secondary lamella is all brownish 
red, and the middle lamella only remains the brighter red with 
