490 
ANNALS OF THE MISSOURI BOTANICAL GARDEN 
[VoL. 3 
series, and toluol was used as an antiseptic in all cases. Each 
experiment was set up in a gas-washing bottle, which was 
fitted with rubber tubes securely stoppered. These washing 
bottles were incubated at 25-30°C. for 20 days, when the am- 
monia was determined by the Folin method. Friedrich’s im- 
proved gas-washing bottles containing 250 ee. of N/50 HCl 
were used for the collection of the ammonia. Air was drawn 
through for 14 hours by means of a Richards’ suction pump, 
TABLE XII 
AMIDASE ACTION IN THE MYCELIUM OF LENZITES SAEPIARIA 
Nitrogen as 
Substrate Form of enzyme material ammonia set} nitrogen 
free set free 
mg. mg. 
ИТИИ Enzyme «іврегвіоп................. 2.10 0.28 
Deae Enzyme dispersion (autoclaved)..... 1,82 |12 ық 
Ше МЕДИЯ ЕРИ Uy trovi rrr tS 1.13 Leite 
Lil ET TEE Maale asee a e E 23.55 22.57 
Lui E EE Meal Mo Gen E 0.98 ^ | ees 
Asparagin..... Enzyme «іврегвіоп................. 1.40 0.00 
Asparagin..... Enzyme Seege (autoclaved)..... ЫМ ` РРР Ре 
Asparagin аіопе|............................... DM Lic 
Asparagin.....| Мег!............................. 0.21 0.07 
Asparagin..... Мен ege T: E Ee 0.00-— |.J. БЕ ree ee 
etam ‚| Enzyme dispersion................. 1.12 0.00 
icetamid : me dn (autoclaved)..... 134 аркасы 
PACOTAIMIC.. 2. 00) МЕД. „а. вовозевввосововововевосе 0.14 0.14 
tamid Е Meal (autoclaved). с. 5. ees 0:98 СТОР H tx 
S o E PETER TERRE TERT E tU EE 0.00 леса 
TABLE XIII 
AMIDASE ACTION IN THE SPOROPHORAL TISSUE OF LENZITES SAEPIARIA 
Nitrogen as 
Substrate Form of enzyme material ammonia se nitrogen 
ee set free 
mg. mg. 
Dl. РРР Enzyme Фөрегвіоп................. 5.46 — 
eee Enzyme dispersion (autoclaved)... .. 6.16 | oS 
Исок S WES ЖӘСЕРІ MMC 646 теве 
2. тен Yer Y Ma Sine SCR EE 18.34 12.18 
Wray cele. ven weal саг deg dir € dE Dis 588. — Без киста 
Asparagin..... Enzyme дізрегвіоп..........:....... 1.89 0.91 
Asparagin..... Enzyme ираса (autoclaved). . 0938 — 15: e 
Asparagin..... JI E 2.24 1.12 
Asparagin..... Meal breng ре Ee зул Лет 
Acetamid...... Enzyme Ерера. ¿71 pap ee 2.03 1.75 
Acetamid...... am dëses (autoclaved)..... 028 WER 
Dum... TL EST 0.28 -- 
cetamid Veal С a a AME EEEE 0.8%. - (aa 
