[VoL. 3 
492 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
latter erystallizes when the petroleum ether is evaporated, and 
the crystals, after recrystallization from water, melted at 
121°C. and had the appearance of benzoic acid. In the con- 
trols no residue was obtained. The hydrolysis in the case of 
Penicillium Camemberti was 76 per cent. 
In 1912 Kossowicz found that enzyme preparations of As- 
pergillus niger, Mucor Boidin, Phytophthora infestans, Isaria 
farinosa, Botrytis Bassiana, and Cladosporium herbarum in 
every case brought about the destruction of hippuric acid. As 
one of the reaction by-products he identified ammonia, but in 
no case did he state in how great quantities the ammonia was 
formed. Reed (713) found that an enzyme powder prepared 
from Glomerella rufomaculans showed the presence of hip- 
puricase after incubation for one week. 
In 1913 Dox and Neidig applied Sórensen's formaldehyde- 
titration method for the determination of the acidity of amino 
acids to the hippuric acid hydrolysis. The method is founded 
on the reaction between formaldehyde and the primary amino 
group. Hippuric acid has no primary amino group, but after 
hydrolysis the primary amino group of the glycocoll split off 
may be neutralized with formaldehyde, leaving the carboxyl 
unchanged to be titrated against an alkali. In this case Dox 
and Neidig used N/10 Ba(OH)s. They grew cultures of As- 
pergillus niger, A. clavatus, A. fumigatus, Penicillium er. 
pansum, P. Roqueforti, and P. Camemberti on a nutrient solu- 
tion. The cultures were grown for 1, 2, 3, and 4 weeks, the 
juice pressed out after grinding the mycelium and used as an 
enzyme preparation. In all of these fungi hippuricase was 
found, as well as in taka-diastase (Aspergillus Oryzae). The 
age of the mycelium had little influence on the production of 
hippuricase. Titrations for free ammonia showed that in the 
cultures 3 and 4 weeks old there were slight amounts, if any, 
of ammonia, and a secondary reaction, or the splitting of 
glycocoll, is improbable. 
Our experiments with the meal from the mycelial and sporo- 
phoral tissues of Lenzites were carried out in the same way 
as those described by Dox (710). Fifty ecubie-centimeter quan- 
tities of the substrate were used, and the flasks were ineubated 
