[Vor. 4 
140 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
placed in a large 3-liter flask containing benzol. To this flask 
was connected a reflux condensor, and the benzol boiled. The 
benzol was changed from time to time until no resin was 
obtained when 100 сс. of the benzol in which the blocks had 
boiled for 10 hours was distilled. These resin-free culture 
blocks were then placed in an electric oven and dried for 10 
days at 65° C. Cultures were prepared in the same way as 
in series A, and the controls and the resin-free blocks were 
segregated. All were inoculated with Lenzites saepiaria. The 
results obtained are given in table п. The percentage of loss 
in weight of the resin-free blocks, of course, is based on the 
weight of the blocks after the resin was extracted. The results 
of the experiment are very striking, for on the average the 
percentage loss in weight is greater in the control blocks con- 
taining resin than in the resin-free blocks. It may be that 
the vigorous and continued boiling in benzol had such an effect 
on the lignin as to prevent decay. However, in series C where 
the blocks were soaked in benzol for a few hours the benzol 
seemed to have no such effect on the growth of the fungus. 
On the other hand, it may be that benzol dissolves out other 
substances which aid in the nutrition of fungi within the 
woody tissues. Time has not permitted a repetition of series 
B, and until such is done we hesitate to place much stress on 
this phase of the work. 
TABLE II (Series В) 
EFFECT ON THE GROWTH OF LENZITES SAEPIARIA OF EXTRACTING BENZOL- 
SOLUBLE SUBSTANCES FROM YELLOW PINE WOOD 
ке АП IV | у | VI ҮП | ҮШ | IX 
Longleaf pine Shortleaf pine Loblolly pine 
(Pinus palustris) (Pinus echinata) (Pinus Taeda) 
Ем Евн ба | Вы [Es ба | Ba | Bez] ба 
S8 | Som! бе| Z8 | Sam) 82 | 28 | Sym] 84 
35 |59%| Б | 55 | Ske] + | 53 | 548 | 55 
H3101 52 | 16.68 | А4301 12 3.74 | А2001 | 10.04 17.4 
H3102 | ( 0.0 A4302 | 0.32 0.0 A2002 | 0.02 0.0 
H3103 | 0.14 0.0 А4303 Al 0.0 А2003 | 0.02 0.0 
H3104 | 0.04 0.0 A4304 | 0.06 0.0 A2004 | 0.09 0.0 
H3105 | 6.42 | 16.68 | А4305 $ 74 | А2005 | 7.56 17.4 
G3201 85 | 24.48 | B4301 24 4.08 | A2101 | 2.28 3.28 
G3202 | 0.88 0.( B4302 | 0.40 0.0 A2102 | 2.92 0.0 
(3203 | 0.15 0.( B4303 | 0.89 0.0 A2103 | 2.93 0.0 
3204 | 0.95 0. B4304 | 0.0 0.0 A2104 | 4.82 0.0 
