[Vor. 4 
170 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
weight at about 105° C., then transferred directly to desic- 
eators with anhydrous and freshly oven-dried CaCl. After 
24 hours they were accurately weighed to the third place and 
marked with weight and number. As a result of the appar- 
ent variations in growth it was determined to make hydrogen 
ion determinations of the solutions, so that under aseptic con- 
ditions the remaining culture fluid was poured off into test- 
tubes for later use, 
Эш на Lupe $H the mycelial mat 
H HH being then thrown 
v 
on to the filter, as 
690 also flask washings. 
The filters were 
soo ВЕ then again dried to 
HFH constant weight at 
Е about 105° C., and 
400 H placed in desicca- 
as tors until carefully 
weighed. 
300 In table т Ше 
average weights of 
the felts from the 
240 
duplicate series 
are given. The re- 
sults in the dupli- 
eate series with 
all decoctions ex- 
cept corn meal 
меге sufficiently 
Fig. 2. Macrosporium commune; dry weights of cons тае 
cultures in mg. on ordinates, solutions (вее р. 166 
for pence) on abscissae. Key to graphs in As mentioned 
fig. 1 above, in the case 
of corn meal it was impossible completely to separate the 
precipitate from the slight mycelial growth, so that the 
figures in the table are somewhat too large, and, as between 
duplicate members, there were weight differences not borne 
out by the record of observations. 
The more important data are likewise strikingly shown in 
соқаны 
