[Vor. 4 
280 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
In combining other nutrients with the standard decoctions 
it is impracticable to make the various solutions in a series 
entirely comparable. It has seemed that the greater error 
would result from any attempt to prepare a double-strength 
decoction, to be diluted with a solution of the nutrients intro- 
duced; so it was determined to add to the decoction the var- 
TABLE I 
GROWTH OF ASPERGILLUS NIGER ON VARIOUS DECOCTIONS WITH AND 
WITHOUT OTHER NUTRIENTS 
Dry weight in grams 
Culture medium Apple |Mangold| Carrot | Celery | Salmon 
1 Natural decoction........... ‚019 „178 .052 .085 .094 
la — dec (to 4- 15 
Füller's scale): ee EE: WEF — .075 . 066 
2 Nat'l. үйе Б ЕЕ 13.68% sugar..| .038 „259 ‚077 ‚489 ‚863 
3 Nat'l. dec’t.+ 13.68% sugar 
Оси 2811 .319 .679 .946 1.063 
4 Nat'l. de ec't. 4- 1% КМО...... .145 .232 .144 .080 .075 
5 Nat'l. dec't. 7-13. А дыл, 
КН:РО............ . 046 .309 .119 .489 .768 
6 Nat'l. dec’t.+ .5% KH;PO, ‚021 ‚233 ‚051 ‚076 „117 
ба Nat'l. Чест. + 1% КН,РО,... 022 240 m Med КЕ 
7 Nat'l. же 13.68% ды. 
+1% КМО:-+.5% KH:PO .462 | 1.016 .922 | 1.257 | 1.028 
8 Nat'l. ль +1% КМО, +. 5% 
ere ee eee .221 .304 131 .079 .085 
9 3 mati. dec' t. and $ Richards’ 
eee eee ie ОЛО ‚445 „547 .537 . 699 „738 
10 2 во. dec’t. апа 1 distilled 
ШАСИ о и РООЛОР ‚016 ‚104 . 028 . 036 .037 
5 nat'l. dec’t. апа 3 Richards’ 
NE А 472: .476 .455 жа ‚469 
$ nat'l. dec't. and $ ash sol...| .025 .137 .034 ТҮ! .057 
Nat'l. dec’t. + .25% MgSO, 
+ 13.68% ѕиваг........... 
ми y t. brought to 107% 
Nat'l, dat brought to 10-6 
Wt .НРО..,........... ‚239 i А 
* The nutrient solution риге Richards solution d in pcm С is та еч 
ир аз зай КМО, 1 gm., KH;PO, .5 gm., MgSO, .25 gm., sugar 5 gm., distilled 
er 100 cc. 
‚235 
ious nutrients in solid form. This necessarily increased 
slightly the volume of the solution. The slight excess over the 
correct volume was, however, discarded, since any increase of 
volume would diminish the area of the flask. Some few tests, 
as indicated in tables 1 and п, required the dilution of the de- 
coction to one-half. In every case the volume of culture 
medium in each Erlenmeyer flask was 25 сс., the cultures were 
made in duplicate, and in certain instances parts of a series 
