ГУ от. 4 
282 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
in the tables by the plus sign, and is to be interpreted that the 
decoction is used as solvent and contains those amounts. 
Where two solutions are mixed half the quantity of each is 
employed, so that the resulting medium is half strength with 
respect to each. 
TABLE III 
ASPERGILLUS AND —— ON RICHARDS' SOLUTION AND 
ODIFIED DECOCTIONS 
| Dry weight in gms. 
Culture medium Asper- | Gloeo- 
gillus |sporium 
11 3 Richards' sol. and $ distilled water.................... .424 .202 
I2/5PISDardat ВОТ sia a OR Soo лы Как vc o EO УЫ .587 ‚343 
13 Modified Richards’ sol. to contain 13.68% sugar + .5% m "m" 
Modified Richards' sol. to contain 13.68% sugar + .25% 
БАЙЫДЫ о ана 755 522 
j па M Раз dec’t. and $ Richard's ПИАРА 457 542 
5 nat'l. bean dec't. and $ bean Чес Г. ash sol.............. 043 044 
4 nat'l. prune dec't. and $ Richards’ sol.................. 456 517 
5 nat'l. prune dec't. and $ prune Чес". ash sol............ 070 082 
3 па! |. sugar beet dec't. and 3 Richards’ sol.............. .437 .498 
3 nat'l. turnip dec't. and 4 Richards’ sol................. 455 ‚501 
$ nat'l. turnip dec't. and $ turnip Чес". ash sol........... ‚044 „051 
Naming the decoctions in the order of the best growth as 
determined by the criterion of weight the series for Asper- 
gillus is as follows: mangold, salmon, celery, carrot, and 
apple; while for Gloeosporium the same order prevails except 
that celery and carrot exchange places. The mangold is rela- 
tively rich in sugar, and this perhaps explains the higher 
growth quantities in the decoction of this product as com- 
pared with the other native decoctions mentioned. The addi- 
tion of sugar alone to mangold decoction, as might be ex- 
pected, gives very little more growth than the addition of 
one of the mineral nutrients. With the addition of sugar 
alone to the various decoctions Aspergillus shows the greatest 
increase in salmon decoction, about 900 per cent, the celery 
medium exhibiting the next greater increase; while with 
Gloeosporiwm the order of these two higher is reversed. Тһе 
marked increase in growth in salmon decoction with the addi- 
tion of sugar is a clear indication that this substratum is well 
provided with the other essential nutrients. The general ap- 
pearance of the curves indicating the yields in celery solutions 
