[Vor. 4 
284 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
ash was then dissolved or diffused in 25 cc. of water and com- 
bined with the decoction. In all cases this addition of mineral 
constituents gave slightly increased yield. 
Aspergillus fruited well in the great majority of cultures. 
In the mangold solutions some depression of fruiting occurred 
45 
700 
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год 
ee: 
300 Қ. 
/ 
/ 
400 
со E о тм ,м 
© е) с "© 
zi & Z В | я d 
т + + + S % 4 9 
© СӨ ж.“ о ЗС -i ы 8 Шш 
8 FI E ZR PF E E P2 8 - B 35 1 3 
аа A а a ^ 4 8 HAA 
ee ee ШЕ ee 
ра тр B i1 B I 3 
ПЕ ПИ Же e D M Ж 
5 в4 4 4 d dd 8 ы о d d d dà 5 
Fig. Growth of Aspergillus and Gloeosporium on mangold decoction 
and other nutrients; Aspergillus, solid line; Gloeosporium, broken line. 
where the nutrients were less well balanced, as where sugar 
and nitrate or sugar and phosphate alone were added. Much 
the same effect was noted in the carrot and celery decoctions, 
though here it was less pronounced. While spore prodali 
was considerable on the various salmon cultures, the general 
appearance of the fruiting surface was gray rather than black. 
