гара 
1918) 
MOORE—GOMONTIA LIGNICOLA, N. 8Р. 217 
visible in the young cells, but as they become more mature 
no pyrenoids can be observed. Simple staining methods 
showed but one nucleus. Later the cells became so packed 
with chlorophyll and starch that it was not possible without 
sectioning to determine satisfactorily whether or not this 
nucleus divided previous to the actual germination of the 
spore. The wall of the resting spore is always decidedly 
thickened at maturity and various lamellate exerescenees and 
protuberances, referred to by other authors as ‘‘rhizoids,’’ 
are not infrequently formed. The spore, after reaching ma- 
ture size, may rest for months or even years. No change in 
nutrient solution or environment seemed to have the slightest 
effect on inducing germination, although almost every con- 
ceivable combination was tried. From time to time certain 
spores, either in pure or mixed cultures, would begin to ger- 
minate and there was no correlation between the results and 
the treatment to which the various cultures had been sub- 
jeeted. 
The germination of the resting spore may apparently take 
place at any point and usually germ tubes push out at more 
than one place on the surface of the cell. Ordinarily these 
do not appear simultaneously but after a filament of several 
cells has been produced a new filament will start at some other 
point and this may be repeated until as many as four new 
filaments arise from a single spore. The resting spore is 
seemingly inexhaustible, retaining its bright color and abun- 
dant starch content until all the filaments produced are of con- 
siderable length. Then it slowly disintegrates, frequently 
leaving remnants of the old spore wall attached to the basal 
cell of the filament. 
APLANOSPORE OR AKINETE? 
In the original account of G. polyrhiza Bornet and Flahault 
describe what they termed aplanospores. These were about 
4 » in diameter, the size of the small zoospore, and their de- 
velopment into large irregular cells which persisted for a long 
time without change is practically the same as described above 
for the resting spore. In fact there can be no doubt that the 
