[Vor. 5 
244 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
some days previously were made to determine the effect of 
varying amounts of chloroform on the conversion of amido- 
nitrogen bodies to protein form. In both cases it was evident 
that such change was inhibited by the anaesthetie, and in 
proportion to the inerease of the concentration employed. 
Respiration experiments in all the following studies were 
made by aspiration of carbon dioxide-free air through the 
experimental chamber into an absorption apparatus. The 
absorption liquid was precipitated with a 5 per cent barium 
ehloride solution, and transferred quantitatively to a vol- 
umetrie flask. After complete precipitation the supernatant 
liquid was titrated with hydrochlorie acid. Nitrogen analyses 
were made by the Kjeldahl method and sugar determinations 
by reduetion of Fehling's solution. 
An attempt was next made in a series of tests to determine 
if hydrolytic processes were associated with the metabolic 
condensations characteristic of the ripening process. The 
materials used were green peas and fresh, green elderberries. 
The data obtained showed that chloroform narcosis reversed 
the course of metabolism in ripening seeds; the narcosis not 
only inhibited to all appearances the elaboration of ‘‘amide’’ 
nitrogen compounds into proteins, but increased the **amide" 
nitrogen as well The condensation or synthesis of the 
simpler sugars into polysaccharides was also inhibited, with 
indications of a reversal of the process. 
A series of experiments with branches of Salix acutifolia, 
potatoes, and other material showed that with respect to in- 
terruption of the rest period the probable predominance of 
metabolic condensation processes over hydrolytic action could 
be altered by etherization, and the period of inactivity sus- 
pended. These experiments were outlined in detail in an- 
other publication of Johannsen and he does not here further 
consider them. 
Etherization of Salix buds (in lots of 200) showed a loss 
in reducing sugar content and a marked gain in nitrogen, as 
compared with the controls. An analysis of fat, sugar, and 
nitrogen relations of green lupine seeds following etheriza- 
tion showed a slight increase of all three products directly 
