1918] 
BONNS—ETHERIZATION AND ENZYME ACTIVITY 267 
For this study corms of a hybrid Gladiolus (G. ganda- 
vensis X 9. psittacinus) were used. These were stripped of 
their coarse outer scales and the basal parts carefully 
scraped clean, without injury to the living tissues. They 
were sorted for uniformity of size and condition and divided 
into four lots of 20 corms each. There was no evidence that 
the corms had started active growth, but it may be assumed 
that at the time they were in the last stages of the resting 
period. The weights of the several lots and their treatment 
follow: 
Lot No. of corms Weight in gms. Treatment 
20 403.2....Etherized for 24 hours 
and extracted. 
403.2... -Control 
, Ее! ZU ie hs РР 402.9....Etherized for 24 hours 
and aired for 18 hours 
before extraction. 
ere ZO e i 401.8. . . . Control. 
Two smaller lots, C and D, of 11 corms each, weighing respec- 
tively 191.7 gms. and 194.3 gms. were also sorted at this time, 
and after disinfection placed in cold storage for future ex- 
periments on catalase action. 
The following substrates were used for a determination of 
the action of the respective enzymes: 
Starch (Merck) 
Dextrin (Merck) 
for amylases 
Sucrose (A. Daigger & Co., h. p.) 
Maltose (Merck) 
Inulin (Merck) 
Ethyl acetate (Sargent & Co., с. p.) | 
for sucrases 
Ethyl butyrate (Sargent & Co., c. p.) for lipases 
Olive oil (Merck) as emulsion 
Asparagin (Merck) 
Acetamid (Merck) 
Albumin (Merck) 
Casein (Baker) 
Peptone (Bacto-peptone) 
Albumin (Merck) in digestion tubes 
for amidases 
for proteases 
