[Vor. 5 
272 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
Series D 1.—Enzyme dispersion from controls extracted 
simultaneously with enzyme of С 1,4 sub- 
strate. 
Series D 2.—Same dispersion, autoclaved before adding to 
substrate. 
In table 1 are given the glucose values obtained by the 
Shaffer method from the several carbohydrate substrates. 
The potassium permanganate solution used in the titration 
was exactly 1/50 normal, 1 cc. being equivalent to 1.272 mg. 
copper. The amount of sugar per 10 се. sample of substrate 
represents the average of two or more titrations,—which did 
not vary by more than .1—2 cc. of the permanganate solu- 
tion,—from which reduction value of the Fehling control has 
been subtracted. 
TABLE I. 
THE ACTION OF ENZYMES EXTRACTED FROM ETHERIZED ium UNETHERIZED 
CORMS OF GLADIOLUS ON CARBOHYDRAT 
Milligrams sugar as glucose 
Substrate Series number 
Con- 
trol. | A 1] A2| B1] B2.1C1]|C2]|D14D2 
Spl.* 13 1,5 1.0 6.6 0.9 2.9 0.7 2.1 0.7 
Starch 
Tot. 
sub.f| 5.8 | 36.6 5.2 | 33.0 4.6 | 14.6 3.4 | 10.6 3.4 
Spl. 6.0 | 21.9 5.9 | 25.6 5.1.1]. 13.1 7.9 | 10.0 | 14.0 
Dextrin 
Tot. 
sub. 30.0 | 109.4 | 29.4 | 128.2 | 25.6 | 65.4 | 39.4 | 49.8 | 70.2 
Spl. yer 11.4 9.6 8.4 8.9 9.0 | 10.6 9.7 | 13.4 
6.4 
Sucrose 
Тов. 121142 57.0 | 48.0 | 42.2 | 44.4 | 45.0 | 52.8 | 48.6 | 67.0 
sub. | 31.81 
Spl. 41.2 | 42.6 | 42.0 | 44.3 | 44.2 | 42.6 | 43.6 | 42.7 | 42.7 
Maltose 
"Dot: 
sub. | 206.2 | 213.2 | 210.0 | 221.6 | 221.0 | 212.8 | 218.0 | 213.4 | 213.4 
Spl. 6.9 6.6 7.4 7.0 6.6 5.8 5.5 5.8 7.8 
Inulin 
Tot. 
sub. 34.4 | 33.2 | 37.0 | 35.2 | 32.8 | 28.8 | 27.4 | 28.8 | 39.2 
* Spl.= amount in sample. 
T Tot. sub.— total in ИР 
1 Control minus aci 
