[Vor. 5 
278 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
time of incubation by the blowing out of the corks and conse- 
quent evaporation. 
TABLE III 
THE ACTION OF ENZYMES EXTRACTED FROM ETHERIZED AND UNETHERIZED 
CORMS OF GLADIOLUS ON PROTEINS 
Milligrams amino-nitrogen from albumin 
Series number Neutral Alkaline 
In In In total 
sample substrate sample substrate 
Control......... .2918 1.294 . 2923 7.307 
ИТТІ .4292 10.730 .8291 20.727 
) o5 APTENT .2951 7.311 ‚3933 9.837 
пр Ж ИТ ТҮК 2307 5.767 ‚4285 10.712 
EX ice 2200 5.500 .2486 6.215 
ER ee warner .3970 9.925 Foer aedi! EU 
Eae. y .2414 6.035 .2232 5.580 
BE Re errr 2993 7.482 .2551 6.378 
DE 2762 6.680 .3455 8.637 
Milligrams amino-nitrogen from 
Casein Peptone 
Series Neutral Alkaline Neutral Alkaline 
number 
In In In total Іп | In total 
sample [substrate sample substrate sample substrate sample substrate 
Control...| .644 .122 | .464 1.605 | .6120 | 15.300 | .6396 | 15.990 
Ди, o3 9797 | 24.492 | .7427 | 18.567 | .8605 | 21.512 0 20.165 
Ж PIT 7249 | 18.1 6790 | 16.975 | .5810 | 14.525 | .5570 | 13.975 
Б ОРУТ 8848 | 22.120 |1.4058 | 35.145 | .9381 | 23.452 | .8545 | 21.362 
РР 9541 | 23.852 | .4774 | 11.935 | .8117 | 20.292 | .8018 | 20.045 
b d ove 7904 .760 | .6631 | 16.577 | .6737 | 16.842 | .9178 | 22.945 
ау ЖАРҒЫ 1.2060*| 30.150*| .4297 | 10.742 | .7056 .640 |1.0022 | 25.055 
DES .742 .567 Pose 7109 | 17.772 | .9534 | 23.835 
52 Mem .8929 | 22.322 | .5835 | 14.587 | .6544 | 16.360 | .7016 | 17.540 
* Autoclaved twice after incubation. 
T Lost by evaporation in incubator. 
The results here vary considerably with the substrate and 
with the reaction of the solution. In the case of albumin a 
definite increase in amino-nitrogen may be noted in the flasks 
acted upon by enzymes from etherized corms, in both halves 
of the series and in alkaline as well as in neutral media. In 
the former the action has been practically twice as great as 
in the neutral albumin. In casein the amino-nitrogen values 
are as a whole considerably higher than for albumin, but a 
comparison of the respective controls shows that this may be 
