1918] 
BONNS—ETHERIZATION AND ENZYME ACTIVITY 285 
the respective flasks would perhaps throw some light upon the 
effect that autoclaving might exert upon the organic matter 
present in the flours, as indicated in the sugar determinations. 
In the table below the following notation is used: 
C.—Substrate with flour from unetherized barley. 
Cf.—Substrate with flour from unetherized barley, flour re- 
moved before autoclaving. 
E.—Substrate with flour from etherized barley. 
Ef.—Substrate with flour from etherized barley, flour removed 
before autoclaving. 
Sb.—Substrate control. 
TABLE VII 
THE ACTION OF FLOUR MADE OF ETHERIZED AND UNETHERIZED GERMINATING 
SEEDS OF HORDEUM ON CARBOHYDRATES 
Milligrams sugar as glucose 
Substrate Series letter 
C Cf E Ef Sb 
Spl.* 23.53 22.19 22.80 21.49 0.38 
h 
Starc Tot. 
sub. 1 117.65 110.95 114.00 107.45 1.90 
Spl. 21:17 21.83 24.76 20.09 8.18 
S 
ucrose Tot. 
sub. 105.60 109.15 123.80 100.45 40.90 
Spl. | 38.63 39.53 37.33 37.82 26.53 
Maltose 
Tot. 
sub. 193.15 197.65 186.65 189.10 132.65 
= amount in sam 
1 "A sub.= total in Mid. 
The sugar data above show a consistently lower value for 
all substrates with flour from etherized seeds, where the sub- 
strate was autoclaved after being separated from the solid 
constituents of the barley. This is also true in most of the 
substrates where the flour and the solutions were heated to- 
gether, but there is a marked deviation in the case of sucrose, 
where E is considerably greater than C. On the other hand, 
Cf, conforming to the relations holding in the other substrates, 
is larger than Ef. It is also evident that the relations of C 
