30 



[Vol. 6 



ANNALS OF THE MISSOURI BOTANICAL GARDEN 



the temperature was 23-25° C, then 28-30° for 3 days, while 

 for the last 5 days of the 10-day incubation period it was 35- 



37 



The rotating machine 



i 



d once in 24 seconds 



After 10 days one-half of the flasks of the shaker set and of the 

 shelf set were subjected to nitrogen analyses, and the other 



half to sugar determinat 

 and viii. 



The results appear in tables vii 



TABLE VIII 



SERIES B— SOLID PHASE REMOVED 



No. 



Treatment 



Condition at close 



Nitrogen 

 (mgs.) 



Shaker 



17 



Check 



18 



Check 



19 



Inoc. 



20 



Inoc. 



Clear 



Clear 



Clear 



Faint turbidity 



0.42 

 0.42 

 0.41 

 0.68 



21 



Check 



22 



Check 



23 



Inoc. 



24 



Inoc. 



Residual sugar 

 (mgs.) 



4 t 



• • 



* 



881. 

 600. 



Shelf 



25 

 26 

 27 



28 



Check 

 Check 



Inoc. 

 Inoc. 



Nitrogen 

 (mgs.) 



Clear 



Clear 



Faint turbidity 



Faint turbidity 



0.42 



Lost 

 1.11 



1.75 



Residual sugar 

 (mgs.) 



959. 

 945. 

 933. 

 914. 



* By mistake these cultures were subjected to nitrogen analyses. Nos. 21 and 

 22 contained 0.393 and 0.418 mgs. N, respectively. 



This experiment brings out very clearly the effect of remov- 

 ing the precipitated phosphates from Ashby's solution. The 

 medium from which this precipitate has been removed is very 

 poorly suited to the support of this microorganism. It seems 



that the shaker is detrimental with this medium, while 

 distinctly beneficial when the precipitate of phosphat 



1 Constructed similar to the one used bv Bonazzi. loc, cit. 



IS 



