1919] 



DODGE — TYROSINASE REACTION 



83 



flasks at the end of the experiment. In all of the flasks the 

 amount of amino-nitrogen lost and not accounted for as am- 

 monia was small and it may probably have conjugated with 



SHOWING LACK OF 



TABLE II 



CORRELATION BETWEEN LOSS OF 

 GAIN OF AMMONIA-NITROGEN 



AMINO-NITROGEN AND 



Solution 



Amino-nitrogen 





Ammonia-nitrogen 



Mgs. present 

 Start End 



Loss 

 (nig.) 





Daedalea confragosa 











1 . 0000 gm. fungous flour 



70 cc. water 



.0835 



.0617 



.0217 



70.4 



.0051 



.0026 



.0025 



8.2 



0. 5000 gm. fungous flour 



75 cc. water 



.0627 



.0507 



.0120 



38.9 



.0021 



.0009 



.0012 



3.7 



0. 2500 gm. fungous flour 



75 cc. water 



.0462 



.0401 



.0061 



19.7 



■ 



.0012 



.0008 



.0004 



1.3 



0. 1250 gm. fungous flour 



.0517 



.0212 



.0305 



98.7 



.0085 



.0037 



.0048 



15.6 



0.0625 gm. fungous flour 



75 cc. water 



.0499 



.0451 



.0048 



15.5 



.0092 



.0030 



.0063 



20.4 



0. 1250 gm. fungous flour 



75 cc, water (35°C.) 



.0502 



.0268 



.0234 



75.7 



.0044 



.0033 



.0011 



3.5 



0. 1250 gm. fungous flour 



75 cc. water (5°C.) * 



.0523 



.0505 



.0018 



5.8 



.0040 



.0037 



.0003 



1.3 



0. 2500 gm. fungous flour 



75 cc. water (35°C.) 



.0438 



.0300 



.0138 



44.6 



.0193 



.0071 



.0122 



39.5 



0. 2500 gm. fungous flour 



75 cc. water (20°C.) 



.0414 



.0210 



.0204 



66.0 



.0075 



.0069 



.0006 



2.2 



0. 2500 gm. fungous flour 



75 cc. water (5°C). 



.0459 



.0434 



| .0025 



8.1 



.0044 



1 .0044 



.00001 0.0 



Armillaria mellea 



0. 250 gm. fungous flour 



75 cc. water (35°C.) 



. 250 gm. fungous flour 



75 cc. water (20°C.) 



. 250 gm. fungous flour 



5 cc. water (5°C). 



0. 250 gm. fungous flour 



75 cc. water 



.0346 



.0284 



.0062 

 .0025 



21.0 



.0052 



.0031 



.0468 



.0443 



8.1 



.0043 



.0028 



.0459 



.0434 



.0025 



8.1 



.0033 



.0033 



.0424 



.0296 



.0128 



41.4 



.0044 



.0018 



.0021 



.0016 



.0000 



.0026 



6.7 



5.1 



0.0 



8.3 



* This was the flask in which the pellicle was noted. The two succeeding sets 

 of flasks yielded bacteria. Probably none of the flasks of solution were wholly 

 sterile (see discussion). 



t Computed on the basis of tyrosin N present at the beginning of the experi- 

 ment, 0.0309 gm. 



