[Vol. 6 



88 



ANNALS OF THE MISSOURI BOTANICAL GARDEN 



trations as usual. Data is lacking as to the possibility of 

 these groups being partly due to compounds such as oxy- 

 phenyl lactic acid which Kotake ('18) thinks is a normal in- 

 termediary product in tyrosin metabolism, but this compound 

 reacts so much more readily than melanin that it seems im- 

 probable that this acid is formed, since deamination is an 

 essential feature of its formation. 



TABLE III 



SHOWING THE AMOUNT OF CARBOXYL AND PHENOL GROUPS 





No. cc. N/50 Ba(OH), for 5 cc. sol 



indicated 



. to give Ph values 



Ph 



VnlllPQ 





Daedalea con 



fragosa 



Armillaria 

 mellea 





f 111 Uv**> 



.0625 

 gm. 



0.125 gm. 



0.250 gm. 



0.250gm. 



Average 





20°C. 



35°C. 



5°C. 



35°C. 



0.12 



0.15 1 



0.17 



0.36 



0.36 



0.38 



0.38 



0.55 



0.60 



0.66 



0.67 

 0.67 

 0.67 

 0.66 

 0.66 

 0.66 

 0.66 

 0.66 

 0.67 

 0.67 

 0.85 



5°C. 





8.0 



0.09 

 0.11 

 0.11 

 0.15 

 0.15 

 0.18 

 0.24 



0.25 

 0.34 



0.17 

 0.22 

 0.35 

 0.36 

 0.36 

 0.46 

 0.66 

 0.79 

 0.79 

 0.79 

 0.87 

 0.91 

 0.94 

 0.96 

 0.97 

 0.97 

 0.98 

 0.98 

 0.98 

 0.99 

 1.00 



0.53 

 0.58 

 0.58 

 0.58 

 0.72 

 0.77 

 0.84 

 0.98 

 1.04 

 1.05 

 1.06 

 1.07 

 1.08 

 1.09 

 1.10 

 1.10 

 1.10 

 1.11 

 1.11 

 1.12 

 1.12 



0.24 

 0.26 

 0.28 

 0.40 

 0.38 

 0.34 

 0.33 

 0.30 

 0.30 

 0.29 

 0.29 

 0.30 

 0.29 

 0.30 

 0.29 

 0.29 

 0.29 

 0.29 



0.00 

 0.01 

 0.03 

 0.15 

 0.26 

 0.30 

 0.50 

 0.52 

 0.53 



0.18 



8.2 



0.21 



8.4 



0.25 



8.6 



0.30 



8.8 



0.35 



9.0 



9.2 



0.39 

 0.50 



9.4 



0.58 



9.6 



0.61 



9.8 



0.71 



10.0 





0.91 

 0.94 

 0.96 

 1.00 

 1.15 

 1.18 

 1.21 



0.76 



10.2 





0.78 



10.4 





0.79 



10.6 





0.80 



10.8 





0.84 



11.0 





0.84 



11.2 





0.85 



11.4 





^^^^ ^ ^^^~ ^^^^ 



11.6 









11.8 









12.0 















All of this work should be repeated under absolutely sterile 

 conditions, if this be possible, before one could be justified 

 in drawing conclusions. However, the data are highly sug- 

 gestive. 



From my work with the cultures of bacteria isolated, I am 

 certain that the characteristic pigment formation of tyrosin 

 is not due to them, as they are unable to produce any color in 

 solutions of tyrosin or tyrosin agar, either with or without 



