112 AXXALS OF THE MISSOURI BOTANICAL GARDEN 



[Vol. 6 



CULTURE RELATIONS: FTXUI 



The three fungi used were selected because of their vigorous 

 growth in cultures, their importance as wood-destroying 

 forms, and the fact that they are found on both coniferous 

 and deciduous hosts. Fomes pinicola Fr. is particularly de- 

 structive on a great variety of conifers. It has also been re- 

 ported by Weir ('14) on Populus trichocarpa, P. tremuloides, 

 Betula occidentalis, Fagus sylvatica, Quercus pendiculata, 

 and other deciduous hosts. Poly si ictus versicolor (L.) Fr. has 

 a wide range of deciduous hosts and has also been reported 

 by Weir ('14) on Larix occidentalis, Pinus monticola, and 

 Pseudotsuga taxifolia. Lenzites saepiaria Fr. has been previ- 

 ously used in this laboratory by Zeller ( '16) with excellent re- 

 sults. It is more important as an organism causing decay of 

 coniferous woods but has also been reported on Populus alba, 

 P. tremuloides, Betula occidentalis, Acer glabrum, and other 

 deciduous hosts by Weir ('14). 



Cultures of Fomes pinicola were made from young sporo- 

 phores by the tissue method first reported by Duggar ( '05) 

 and later used by Zeller ( '16) in obtaining cultures of Lenzites 

 saepiaria. Young sporophores, from four to six inches in 

 diameter, were washed with tap water followed by a thorough 

 washing with sterile distilled water, and then dried with sterile 

 tissue towelling. An incision from one-half to one centimeter 

 deep was made across both surfaces of the sporophore with a 

 sterile scalpel and the sporophore then broken open. This 

 eliminated the possibility of contamination of the deeper tissue 

 by the carrying down by the scalpel of bacteria and spores 

 from the surface of the sporophore and also left a jagged sur- 

 face on the broken deeper tissues, portions of which were 

 easily torn off by means of a sterile forceps and quickly trans- 

 ferred to Thaxter's hard agar slants. The fungus grew ex- 

 ceptionally well on this media. Twentv-four tissue transfers 



ade, of which twentv-three errew. none be 



nated. 



Cultures of Lenzites and Polystictus were made by the spore 

 method. This method has been extensively used by Falck 



Lvm 



The 



