130 



ANNALS OF THE MISSOURI BOTANICAL GARDEN 



[Vol. 6 



TABLE XVII (Continued) 



I 



II 



III 



IV 



V 



I 



II 



III 



IV 



V 



<D 



o 



o 



u 

 3 



3 



u 



CO ^ 



"0 co 



O 0) 

 U) <D 



•53 -^ 



E 



bo 



£ 



co 



3 

 co <D 



o H 



CO 



E 



1) 



£ 



bo cj 



a o 



CO 



E 



co cL> 

 CO 3 



cu 



CO 

 CO 



O 



72 « 







a 



0) 



o 



CD 



u 



3 



3 



u 



S 



CO 



3 



a; 



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CO 



E 



£ 



CO 



3 



co a; OT - 



CD 



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 !> T; co 



E 



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co qj 

 co « 



J3' 



2 



a) >, a 



ID u u 



m V U 

 $T> JD 



CI 



P. versicolor 2/5/1 7 r Azotobacter 

 ococcum 4/2/17 



c/irc9- 



651 



7.79 



653* 



8.18 



654* 



8.46 



656 



8.53 



657* 



9.95 



659 



7.09 



660 



7.29 



7.78 

 8.02 

 8.35 

 7.51 

 9.75 

 7.07 

 7.25 



0.01 

 0.16 

 0.11 

 0.02 

 0.20 

 0.02 

 0.04 



13 



1 96 

 1 30 

 0.23 



2.01 

 0.28 

 55 



P. versicolor 2/5/17, B. vulgatus & 

 mycoides 3/31/17 



Bact. 



951 

 952* 



954 f 



955 



956 



957* 



959 



960 



8.88 

 7.59 

 8.59 

 8.09 

 7.01 

 7.16 

 7.41 

 7.80 



8.75 

 7.53 

 7.54 

 8.03 

 6.91 



7.06 

 7.29 

 7.71 



0.13 

 0.06 

 0.05 

 0.06 

 0.10 

 0.10 

 0.12 

 0.09 



1.46 

 0.79 

 0.58 

 0.74 

 1.56 

 1.39 

 1.62 

 1.15 



Average 



92 



Average 



1.16 



* No bacteria present after incubation. 



t Bacterium mycoides present after incubation. 



The average results of the foregoing series are tabulated 



table 



THE GROWTH OF PURE CULTURES OF BACTERIA ON SAWDUST AND 

 THEIR EFFECT ON THE REACTION OF THE SAWDUST EXTRACT 



Due to the fact that in most of the cultures bacteria were no 

 longer found alive at the end of the period of incubation, pure 

 cultures of the organisms were grown on sawdust in order to 

 determine whether or not the sawdust was toxic and the 

 effect, if any, of the bacteria on the reaction of the extract. 



Equal amounts, 5.00 gms., of sawdust were placed in 125-cc. 

 Erlenmeyer flasks and 50 cc. of distilled water were added to 

 each. The flasks were plugged and autoclaved for one hour 

 and subsequently inoculated with various species of bacteria. 

 After thirty days' incubation at room temperature transfers 

 were made from the flasks to hard potato agar, and the reac- 

 tion of the wood extract determined by titrating 5 cc. of the 

 extracts against N/20 sodium hydroxide. Red oak, western 

 hemlock, and Douglas fir were used in this work, with Bacillus 



