[Vol. 6 



166 



ANNALS OP THE MISSOURI BOTANICAL GARDEN 







TABLE IV 







DISINFECTION OF SEED (AFTER IMMERSION IN WATER FOR 4 HOURS) BY CHLO- 



RINATED LIME. FINAL OBSERVATIONS AFTER 96 HOURS 







Interval of 







Kind of seed 



% Concentra- 



• 



treatment, 



% Germina- 



• 



% Contami- 



• 



^^^* ^^*- ^^ m ^» ^^r • ^fc^^ ^™ W^^ ^fc»^ ^fc^' ^^ta^^^ 



tion 

 10 



hours 



tion 



nation 





2 



72 









10 



1 



90 







Corn 



10 

 5 



3 



90 



72 





 2 





5 



2 



84 









5 



1 



86 



6 





10 



2 



12 









10 



1 



24 







Peas 



10 

 5 



* 

 3 



18 

 38 



4 

 





5 



2 



24 



6 





5 

 10 



1 



2 



32 

 84 



4 











10 



1 



84 







Cucumber 



10 



i 



68 



2 





5 



3 



64 









5 



2 



82 



8 





5 

 10 



1 



84 



88 





2 



12 



18 





10 



1 



30 



14 



Radish 



10 

 5 



i 



3 



3 



. 38 

 4 



14 

 56 





' 5 



2 



8 



58 





5 



1 



28 



24 



tion (not perfect in the case of radish) where the potassium 

 salt was employed, mark this as far more reliable under these 

 conditions. 



During the progress of this work other investigations were 

 in progress by one of us and by graduate students, in which 

 germinating seed free of contaminating organisms were re- 

 quired. The Javel treatment was employed, and with the con- 

 sent of those who cooperated in this work, Dr. W. W. Bonns 

 and Mr. T. Matsumoto, we are enabled to report the following 

 facts : Of 500 seed of Canada field peas treated 3 hours with 

 a 10 per cent solution the germination was 90 per cent and the 

 contamination less than 1 per cent. In treating tobacco seed, 

 2- and 3-hour intervals proved inadequate ; but a 4-hour inter- 

 val with 12 per cent Javel water gave a contamination of less 



