[Vol. 6 



168 



ANNALS OF THE MISSOURI BOTANICAL GARDEN 



TABLE VI 



DISINFECTION OF SEED (AFTER IMMERSION IN WATER FOR 4 HOURS AT 23° C.) BY 



CHLORAZENE, CHLORINATED POTASH, AND SODIUM HYPOCHLORITE. 



FINAL OBSERVATIONS AFTER 96 HOURS 



Disinfectant 



1% chlorazene 



Kind of seed 



20% chlor. 

 potash 



Corn 

 Peas 



Cucumber 

 Radish 



Corn 

 Peas 



Cucumber 

 Radish 



20% sodium- 

 hypochlorite 



Corn 

 Peas 



Cucumber 

 Radish 



% Germi 

 nation 



% Contami 

 nation 



100 

 26 



100 

 40 



96 

 94 



84 

 64 



100 

 22 

 96 

 12 



50 

 100 



100 





 





 16 



2 



50 











Condition 



Good 

 Good 

 Injured 

 Good 



Good 

 Good 

 Good 

 Good 



Good 

 Injured 

 Good 

 Good 



tion, as well as an unusually high percentage germination as 

 compared with the usual percentage when the same seed are 

 treated with any such disinfecting agent and placed directly 

 on agar. The principle involved may possibly be widely ap- 

 plicable and deserves consideration in other work. 



The results of similar tests with chlorinated lime, varying, 

 however, the time intervals and the concentration of the dis- 

 infectant, are given in table vm. 



In addition to the results reported in full, extensive experi- 

 ments were made with sodium chlorate and calcium sulphite. 

 Concentrations of 10 and 5 per cent of the pure reagents were 

 employed for various intervals, but in all cases 100 per cent 



TABLE VII 



DISINFECTION OF SEED BY 20 PER CENT CHLORINATED POTASH,— DISCONTINUOUS 



TREATMENT. FINAL OBSERVATIONS AFTER 96 HOURS 



Kind of seed 



Corn 



Peas 



Cucumber 



Radish 



% Germination 



100 



90 



100 



50 



% Contamination 















20 



Condition 



Fine 

 Good 

 Fine 

 Fair 



