196 



ANNALS OF THE MISSOURI BOTANICAL 



(Vol. 6 



distilled water were used as substrat 



After the enzyme 



culture had been incubated for 28 days there was a marked 

 crease in the amounts of reducing sugars produced by all the 

 fungi on all of the substrates. 



For a hemicellulose substrate cleaned autoclaved endosperms 

 from date seeds were used. These were shaved into very thin 

 slices and placed in distilled water with the fungous meal. 

 Hydrolysis quite comparable to that produced in the cellulose 

 experiments resulted in each case as is indicated in table i. 



Emulsin 



The presence or absence of emulsin was determined by the 

 effect of the fungous meal upon amygdalin, which upon hydroly- 



sis prod 



glucose, benzaldehyde, and hyd 



acid 



After incubation of seven days the amount of w 



the cultures was determined as in the cases where carbohydrates 



used as substrat 

 table 



tabulated 



TABLE II 



ACTION OF EMULSIN ON POLYPORUS LUC] 

 MELLEA, AND DAEDALEA CONFRAGOSA 



Enzyme culture 



1\ lucidus 



A. mcllea 



D. confragosa 



N 



Nuinhor of cc. of— KMnO, 



20 



1% amygdalin -f 

 fungous meal 



1% amygdalin + 

 fungous meal (autoclaved) 



1% amygdalin 



2(3 . 



12.5 



4.8 



0.6 



0.6 



0.6 



18.1 



0.1 



0.6 



d evidence 



breaking down of amygdalin the odor of benzaldehyde 



nized 

 used. 



dence of emulsin in all three of 



Tannase 

 determine the tannase activity the gallic acid 



In order to 

 hich is a product of hydrolysis of tannic acid 



