1919] 



SCHMITZ AND ZELLER — ENZYME ACTION IN CERTAIN FUNGI 199 



presence of trypsin when albumin of acid reaction was used 

 This was not true of Armillaria mellea or Daedalea confragosa 

 In no case was there a splitting of peptone or casein. 



TABLE III 



UREASE AND AMIDASE ACTIVITY IN ARMILLARIA MELLEA, DAEDALEA 



CONFRAGOSA, AND POLYPORUS LUCIDUS 





Enzyme 

 culture 



No. 



Change in H ion concentration. 



P H values 



• 





Urea 



Acetamid 



Asparagin 



* 





Substrate -f 

 fungous meal 



1 



2 

 3 

 4 

 1 

 2 

 3 

 4 

 1 

 2 

 3 

 4 



1 



2 



5.6-6.8 

 3 min. 



5.6^5.6 

 3 min. 



5.6^5.6 

 3 min. 



P. lucidus 



5.6^6.6 

 3 min. 



5.6^5.6 

 3 min. 



5.6-5.6 

 3 min. 



Substrate -f- 



fungous meal 



(autoclaved) 



5.6-^.0 

 3 min. 



5.6-5.6 

 3 min. 



5.6-5.6 

 3 min. 



* 



5.6^5.8 

 3 min. 



5.6-5.6 



3 min. 



5.6-5.6 

 3 min. 





Substrate + 

 fungous meal 



5.6-7.2 

 30 sec. 



5.6^6.0 

 3 min. 



5.6^5.6 

 3 min. 



A. mellea 



5.6-7.2 

 25 sec. 



5.6^6.0 

 3 min. 



5.6^5.6 

 3 min. 



Substrate + 



fungous meal 



(autoclaved) 



5.6^6.0 

 3 min. 



5.6-5.6 

 3 min. 



5.6^5.6 

 3 min. 





■ ■ 



5.6^6.0 

 3 min. 



5.6-5.6 

 3 min. 



5.6^5.6 

 3 min. 





Substrate + 

 fungous meal 



5.6-7.2 

 1 sec. 



5.676.6 



3 min. 







5.6 5.6 

 3 min. 



i 



D. confragosa 



5.6-8.8 

 3 sec. 



5.6-5.6 

 3 min. 



5.6-5.6 

 3 min. 



Substrate + 



fungous meal 



(autoclaved) 



5.6^6.0 

 3 min. 



5.6-5.6 

 3 min. 



5.6-5.6 

 3 min. 





5.6-^.0 

 3 min. 



5.6^5.6 

 3 min. 



5.6^5.6 



3 min. 







Control 



Substrate 

 alone 



5.6^6.0 

 3 min. 



5.6^5.6 

 3 min. 



5.6^5.6 

 3 min. 



* 



5.6^6.0 

 3 min. 



5.6^5.6 

 3 min. 



5.6^5.6 

 3 min. 



