310 



Vol. 6 



ANNALS OF THE MISSOURI BOTANICAL GARDEN 



Since additional cultural results with Melampsora 

 f Salix and Populus have been secured since the Dosi 



mentioned above, suDulem 



the 



Melampsora on Salix IIrbaniana v. Seem. 



In April, 1916, a large number of inoculations of Larix decidua 

 with Melampsora obtained from Salix Urbaniana were under- 

 taken for the purpose of verifying the results published in my 

 earlier paper. Positive results were readily secured on Larix 

 decidua, as shown in table i, while on the remaining species the 

 inoculations were unsuccessful. 



In May, 1916, several series of infection experiments were 

 performed by the inoculation of Salix Urbaniana with the 



caeomaspores 



hi 



had be 



duced on Larix decidua. 



eek positive results were secured 



TABLE I 



SHOWING the results of inoculation with melampsora 



FROM SALIX URBANIANA 



Inoculation 

 material 



Species 

 inoculated 



D ite of 

 inoculation 



Result 



Date of 

 first sori 



Teleutospores 

 from 

 Salix Urbaniana 

 Salix Urbaniana 

 Salix Urbaniana 

 Salix Urbaniana 



Larix decidua 

 Salix Urbaniana 

 Allium Cepa 

 Chelidonium 

 majus 



Caeomaspores 

 from 

 Larix decidua 



April 28 



April 28 

 April 28 

 April 28 



+ 



May 15 



Salix Urbaniana 



M*,y 18 



+ 



V • 



May 25 



From the experiments it is evidently established that the 

 species on Salix Urbaniana found in Sapporo, Japan, is heteroe- 

 cious and must have its aecidial stage on Larix sps. In consid- 

 eration of the evidence given, as well as that which follows, I 

 consider this a new species, and the accompanying diagnosis 

 and notes are offered : 





