1920] 
ALLEN—CARBOHYDRATE CONSUMPTION BY AZOTOBACTER 77 
rotator, and a lesser one in the Erlenmeyers on the shelf. At 
short intervals 5-cc. samples were removed aseptically and sub- 
mitted to duplicate sugar determinations by the modified 
Shaffer method described in the previous paper (Allen, 19). 
Cultures II and IV received on March 24, 25 cc. of an 8 per 
cent dextrose solution. Culture No. III was unfortunately 
lost at this point. 
The data are reported as dextrose remaining per 100 cc. of 
culture solution, no correction being made for evaporation; that 
is, if a 5-cc. sample is found to contain 35 mgs., the value 
reported is 0.7000 gm., regardless of the total volume of the 
culture at the time. 
The results appear in tabular form in tables 1 and 1, and in 
graphical form in fig. 1. 
TABLE I 
NUMBER GRAMS RESIDUAL DEXTROSE PER 100 CC. CULTURE SOLUTION AT 
DIFFERENT PERIODS 
Culture no. | March 17 | March 19 March 24 | March 28 | April 2 
Rotator: (round-bottomed flasks) 
I (Check) 2.1565 
II 1.2732 0.7600 0.110-2.060 1.976 1.255 
III 1.4822 1.3507 0.908-Lost 
IV 0.9410 0.5700 0.069-2 .023 1.842 1.358 
Shelf: (Erlenmeyer flasks) 
V 1.7257 1.636 1.674 1.523 
VI 1.5962 1.418 1.507 1.389 
TABLE II 
MILLIGRAMS DEXTROSE CONSUMED PER DAY IN EACH PERIOD 
Culture First Next Next Additional | Additional 
3 days 2 days 5 days 4 days 5 days 
I Check 
II 295 256 130 21 144 
III 225 65 88 
IV 405 185 100 45 97 
