(Vor. 7 
276 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
TABLE VIII 
GROWTH AND RA Gadus ION CONCENTRATION OF BACILLUS COLI AT 
ree IN INITIAL pet 
ASPARAGIN BOUILLO 
Cultures 1 2 3 
Dextrose 1.0% 2.5% 5.0% 
Hours Bacteria Pu Bacteria Pu Bacteria Pu 
0 45,000 6.6 63,000 6.4 46,000 | 6.4 
12 5,450,000 6.6 100,000 6.4 60,000 6:3 
16 46,500,000 6.5 80,000 6.3 60,000 0.2 
20 326,000,000 5.7 80,000 5.8 60,000 5.9 
24 350,000,000 5.5 145,000 5.7 25,200 5.9 
36 464,000,000 5.4 17,000,000 5.8 8,400 5.9 
48 416,000,000 4.9 345,000,000 5.6 57,600 5.8 
60 280,000,000 4.9 384,000,000 4.9 1,700,000 5.7 
72 178,000,000 | 5.3 | 323,000,000 | 4.9 | 40,000,000 | 5.7 
84 108,000,000 5.3 348,000,000 4.9 170,000,000 5.1 
96 51,000,000 5.3 320,000,000 4.9 270,000,000 4.9 
108 28,000,000 5.3 180,000,000 4.9 260,000,000 4.9 
120 1,800,000 5.3 187,000,000 4.9 380,000,000 4.7 
132 2,800,000 5.3 120,000,000 4.9 224,000,000 4.7 
144 200,000 31,000,000 142,000,000 4.7 
156 18,400 5.3 24,000,000 4.9 161,000,000 4.9 
168 1,000 | 5.3 6,000,000 | 5.2 | 158,000,000 | 5.1 
180 346 5.8 2,000,000 5.2 132,000,000 0:1 
192 144 5.8 360,000 5.0 102,000,000 4.9 
204 93 62,000 104,000,000 
216 391 5.2 54,000,000 5.3 
240 21,000,000 | 5.3 
264 9,900,000 5.0 
300 900 5.0 
Some points of interest in the Pa curves are that the high point, 
Py 4.7, at 120 hours, did not occur during the period of greatest 
decrease in growth, that the P, held at 4.9 for a considerable 
period after the second increase in growth began, and that 
the hydrogen ion concentration at the time when the maximum 
growth was reached was Py 7.1. In plain bouillon there was 
no essential differenee in growth or changes in hydrogen ion 
