[Vor. 8 
12 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
Alternaria, Botrytis, and Penicillium, etc., was much less in- 
hibited on corn meal agar at low temperature than on the apple. 
In 1918 Hemmi, working with a large number of species of 
Gloeosporium, obtained from many different host plants, reported 
a similar relation concerning the maximum temperature of the 
strain isolated from loquat (Eriobotrya japonica). It is clear, 
therefore, that in any study of the temperature relations of fungi, 
it is very necessary to take into consideration all environmental 
faetors, and comparative studies should always be performed 
under the same physiological conditions. 
In cultures on potato agar it is observed that there is little 
difference in the rate of growth of the mycelium of B1 between 
27 and 33° C., and the optimum temperature lies within this 
range, perhaps actually about 31? C. As no growth takes place 
at 13-15" C., the minimum is relatively higher. Above 33? C. 
the rate of growth of the same strain gradually decreases, and in 
most cases, according to my experiments, no new growth is 
secured at 44°C., showing that this temperature is about the 
maximum, or perhaps 42-44°C. 
Almost the same results are obtained in the germination 
experiments with the sclerotia of B1. The germination of the 
sclerotia was studied in distilled water by the hanging-drop 
method at 34-36? C. to 22-24? C. After a few hours the scele- 
rotia generally began to germinate and at the higher temperature 
a large number germinated, while at the lower a few only ger- 
inated, as shown in table rr. 
TABLE II 
EXTENT OF GROWTH OF B1 AT DIFFERENT TEMPERATURES 
Temperature After 4 hours After 8 hours After 15 hours 
34-36? C. 24u 89u About 140. 
22-24? C. No growth 38u About 95u 
In general, the three strains, P1, P4, and H, have about the 
same rate of growth at the different temperatures, although P4 
differs slightly from the other 2 strains. The optimum temper- 
ature of these strains would seem to be about 24° C. At 14-15° 
C. there is still some new mycelial growth, so that the minimum 
