[Vor. 8 
20 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
8 days the experiment was discontinued and dry weights of the 
mycelial felt were obtained (see table v). The second part of 
this table represents a repetition of the work, using also Scle- 
rotinia libertiana by comparison. 
In the next experiments 2 per cent soluble starch was dissolved 
in the stock solution and 50 cc. of the solution used in the Erlen- 
meyer flasks as before. In this case, however, 3 flasks were 
used with each organism. 
| TABLE VI 
SHOWING THE RESULT OF MYCELIAL GROWTH ON SYNTHESIZED CULTURE 
MEDIA 
Period of Reducing 
Strain exp. Pa Growth sugar* Starch 
(days) (mgs.) remaining 
P1 6 5.8 3 1.5 2 
P4 6 6.0 2 5.4 0 
PI 6 5.8 3 1.6 2 
B1 6 6.4 1 1.0 3 
H 6 5.8 3 1.6 2 
B3 6 6.8 1 0.7 3 
Scl. lib 6 6.8 1 0.4 E! 
Control 6 6.8 0 .0 3 
Fi 9 5.6 4 1.6 1 
P4 9 5.7 3 5.8 0 
P7 9 5.8 4 1.7 1 
B1 9 5.9 2 3.9 2 
H 9 5.7 4 1.6 1 
B3 9 6.7 1 1.0 1 
Scl. lib. 9 6.5 2 1.8 1 
Control 9 6.7 0 .0 0 
* Reducing sugar as glucose in 10 cc. of medium. 
From the tables it is to be inferred that all the strains used 
possess diastase, but no quantitative determinations of the 
amount of sugar produced could be made, since constant utili- 
zation of the sugar occurs. 
It is also demonstrated by these experiments that all the 
strains studied have a general tendency to increase the active 
acidity during growth, and the rate of its increase is somewhat 
proportional to the amount of mycelium. 
