1921] 
MATSUMOTO—SPECIALIZATION IN RHIZOCTONIA 21 
Enzyme study—For the purpose of estimating the enzymatic 
activity of these strains, the following experiments were carried 
out by using mycelial extractions added to the substrata to be 
studied. For sugar estimation the method described by Shaffer 
(14) is employed, with slight modification. Two per cent starch 
solution was prepared, and to 20 cc. of this solution there was 
TABLE VII 
A QUANTITATIVE STUDY OF DIASTATIC ACTIVITY IN MYCELIAL AND 
SCLEROTIAL STAGES 
Reducing sugar as glucose in 10 cc. substrate (mgs.)* 
Stage of 
growth 
P1 P4 P7 H B3 
Mycelium 2.54 7.44 2.14 5.95 2.23 
Sclerotia 1.98 6.89 2.15 4.84 2.27 
* Unless otherwise stated, the amounts of reducing sugar are thus reckoned in 
all subsequent tables. 
TABLE VIII 
DIASTATIC ACTIVITY OF THE DIFFERENT STRAINS 
Reducing sugar (mgs.) Diastatic activity (rel.) 
Strain 
After 21 hrs. | After 48 hrs. | After 21hrs. | After 48 hrs. 
P1 7.84 US 30.45 
P4 25.75 58.41 100.00 100.00 
P7. 5.88 9.80 22.83 16.78 
B1 24.69 41.43 95.88 70.93 
H 5.88 9.80 22.83 16.78 
B3 11.86 14.90 46.06 25.51 
Control* Negl. Negl. 0 
PE 12.35 18.95 63.50 64.38 
P4 19.45 29.44 100.00 100.00 
PE 10.78 17.90 55.42 60.80 
B1 13.84 18.95 71.1 64.37 
Control Negl. Negl. 
* Starch solution. 
