[Vor. 8 
24 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
For quantitative estimation of maltose activity a 1 per cent 
solution of maltose was used as a substrate. Twenty cc. of this 
solution were placed in each of 13 test-tubes, 2 tubes for each 
organism and 1 for the control. Five cc. of the extractions 
(0.5 per cent) of the mycelium of the 6 strains were added to 
each tube except the control which received 5 cc. of distilled 
water. All the tubes were placed in an incubator (Ca. 28? C.). 
TABLE XI 
GROWTH OF THE 6 STRAINS ON MALTOSE AND LACTOSE SOLUTION. THE 
QUANTITIES REPRESENT DRY WEIGHT OF MYCELIUM IN GRAMS 
Pa pi P4 P7 B1 H B3 
Maltose 6.8 0.035 0.285 0.237 Negl. 0.330 | Negl. 
Lactose ? 0.140 0.035 0.065 Negl. | ..... Negl. 
Dextrose 7.0 0.332 0.025 0.210 Negl. 0.307 | Negl. 
TABLE XII 
MALTASE ACTIVITY OF THE STRAINS 
Strai Reducing sugar as glucose in 10 cc. substrate (mgs.) 
imn After 6 days After 12 days 
P1 52.68 71.45 
P4 55.44 72.48 
P7 49.58 70.10 
B1 58.48 79.22 
H 52.95 72.15 
B3 52.68 72.15 
Control 47.12 48.11 
No doubt exists concerning the presence of maltase in all 
these forms of the fungus, but as shown by the table, no marked 
specialization is noticed in any of the strains. In this connec- 
tion it is rather interesting to note that the maltose secretion by 
the fungi in question bears a close relation to the nutrient solu- 
tion in which the fungi are grown. In all the strains maltase is 
produced in greater amounts when maltose is used in the cul- 
ture media. It seems to me, however, that there is no ‘‘quali- 
tative” relation of maltase secretion in these fungi, since under 
