[Vor. 8 
28 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
work of McBeth and Scales (713), Zeller (16), and Schmitz (^19), 
therefore no literature review of this subject will be given here. 
Filter-paper cellulose was prepared in the manner described 
by McBeth and Scales (13). Cellulose agar was prepared by 
adding to the stock solution about 1 per cent of the precipitated 
cellulose and 2 per cent agar, afterwards sterilizing as usual. 
Test-tubes were then prepared with about 15 cc. of the solution 
and after autoclaving all the tubes were cooled without being 
slanted, then inoculated. 
All the fungi grew relatively well on this medium, dissolving 
large quantities of cellulose. 'The hydrolysis of cellulose was 
shown by the fact that the agar became transparent over an area 
extending considerably beyond the hyphae. 
TABLE XVII 
GROWTH OF THE STRAINS ON SYNTHESIZED CELLULOSE CULTURE MEDIA 
THE DECIMAL QUANTITIES REPRESENT DRY WEIGHT IN GRAMS 
Cellulose Maltose No carbon 
Strains 
Myecl. Scl.* Mycl. Scl. Myel. Sel. 
P1 0.123 1 0.255 3 0.010 0 
P4 0.062 1 0.252 3 0.009 0 
P7 0.098 0 0.275 0 0.015 0 
B1 0.090 7 0.245 3 0.002 0 
H 0.048 1 0.245 3 0.012 0 
B3 None 0 None 0 None 0 
*The numerals in this and in subsequent tables represent relative amounts, 
1 being the AN PORA amount, in this case being the minimum of positive 
sclerotial formatio 
For a determination of any specialization of the forms in 
respect to cellulose utilization recourse was had to a liquid cul- 
ture medium. This medium was also prepared by adding about 
l per cent of the precipitated cellulose to a complete mineral 
nutrient solution, the cellulose being the only source of carbon. 
For comparison cultures were made with 1 per cent maltose and 
also with the salt solution lacking all carbohydrate. All cultures 
were incubated for 1 month. 
