1921] 
MATSUMOTO—SPECIALIZATION IN F IIZOCTONIA 29 
Direct determination of cellulase was made by employing the 
method described by Zeller (16). Five-cc. quantities of my- 
celial extraction (0.5 per cent strength) were added to 10 cc. of 
the precipitated cellulose solution (about 1 per cent by weight), 
and the result was determined after 1 month. 
TABLE XVIII 
QUANTITATIVE DETERMINATION OF SUGARS RESULTING FROM THE 
CELLULASE ACTIVITY OF THE DIFFERENT STRAINS 
Reduction of Fehling’s solution 
P1 P4 P7 Bl H 
A. Enzyme 3 2 2 2 3 
B. Enzyme (autoclaved) 1 1 1 1 1 
C. Cellulose alone 0 0 0 0 0 
In the light of these results there is no doubt that cellulase 
is present in the mycelium of the strains studied. Its activity 
was not measured quantitatively, but the cellulase activity of 
P1 and H is striking as compared with that of the remaining 
forms. 
The 6 strains were grown in Erlenmeyer culture flasks on a 
mineral nutrient solution containing sucrose and lactose in 
amounts ranging from 0.1 to 10 per cent. Using the dry weight 
of mycelium as a criterion there was, as might be generally ex- 
pected, a progressive increase in yield in all cases except one, up 
to 5 per cent, of both the sucrose and lactose series, after a growth 
period of 1 month. The concentrations from 5 to 10 per cent 
represented a distinct series growing for a period of 5 weeks, and 
here too there was in all cases with increasing concentration a 
progressive increase in growth in the sucrose media, the maximum 
growth occurring in P1 and B1, 0.020 and 1.100 gms. respectively. 
In the lactose media growth at the higher concentrations was 
more or less variable, but the surprising feature of these experi- 
ments was the high yields obtained at 5 per cent or more, the 
maximum being 1.05 gms. in the H strain. In general, the 
limiting concentrations for growth were not determined. 
