(Vor. 8 
56 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
2. The temperature requirements of P1 and H are similar, 
while the remaining strains exhibit different optima, minima, 
and maxima. 
3. All the strains hydrolyze starch, but the diastatie activity 
is unlike. The activity of P4 is notable and stands above that 
of the others. B1 has the next higher capacity, and P1 and P7 the 
minimum. 
4. All these fungi are able to convert cane sugar. This in- 
verting activity of P4, P7, and Bl is striking and many times that 
of P1 
5. Maltase and lactase activity was qualitatively and quanti- 
tatively determined, but insufficient data have been collected 
to determine the extent of specialization. The nutritive value of 
lactose is markedly less than that of maltose. 
6. All the fungi are unable to utilize inulin. 
7. Glucose, fructose, and galactose are utilized by these fungi. 
The availability of these sugars is about the same, and no marked 
specialization was noticed in any of the strains. 
8. Amygdalin is utilized as a source of carbon, being, of 
course, first decomposed by emulsin before becoming available. 
No marked difference in emulsin activity was observed in any 
of the strains studied. 
9. Cellulase is present in the mycelium of these strains. Its 
activity was not measured quantitatively, but qualitative studies 
indicate that it is highest in P1 and H. 
10. P1 and H grow best on casein, peptone, and asparagin 
as sources of nitrogen and carbon, and less well on legumin, 
while P7 grows best on peptone, legumin, casein, and asparagin, 
in the order named. P4 grows best, and equally well on peptone 
and casein, and it grows less on legumin and asparagin. 
11. Potassium nitrate, ammonium sulphate, and ammonium 
nitrate are available as sources of nitrogen, though potassium 
nitrate is preferable. 
12. P1 and H utilize potassium nitrite, while the remaining 
strains cannot do so. Reduction of nitrate is observed in the 
potassium nitrate culture by P1 and H, also P4 and Bl. Abso- 
lutely no reduction takes place in P7. 
13. As a whole, the mycelial growth is more sensitive to 
modification in the carbohydrate supply than to changes in the 
nitrogen supply. 
