[Vor. 8 
72 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
were taken as representative of these conditions. Duggar, 
Severy, and Schmitz (17) have shown that C. Gossypii is a form 
which produces growth on media having an alkaline reaction 
and also that it shifts towards the alkaline side the reaction of 
certain sugar-containing media upon which it is growing. The 
fact that P. italicum grows abundantly upon citrus fruits whose 
reaction varies from Py 2.2 to 4.1 seemed to indicate that it was 
an organism which could be used to represent activities occurring 
on the acid side. The culture of Fusarium sp. was isolated from 
cotton and is the same organism as used by Webb (19). He 
found that the spores had a wide range for germination in re- 
lation to H-ion concentration, varying from Py 2.8 to 10+ in 
the NaOH-H,PO,-mannite solution used. By employing these 
forms, it was thought that the amylase formation under different 
H-ion concentrations could be studied. 
The cultures from which spores were obtained for the subse- 
quent inoculations were on media which produced abundant 
sporulation at room temperature. A synthetic medium pre- 
pared according to the following formula’ was used for C. Gos- 
sypit. 
PRE Riek —— — wo 25 gms 
IMPO. 25 gms 
WE LL sopa Vee eds 10.0 gms 
Glucose ..20.0 gms 
ABE Uni pr (a d reer 15.0 gms 
Water .......1000 ce. 
the method described by Duggar, Severy, and Schmitz (17), 
while Czapek's solution containing starch as the source of energy 
and 1.5 per cent agar was found to produce abundant spores in 
the case of P. italicum. 
Spore suspenston—The spores used in making the subse- 
quent inoculations for the enzyme studies were from cultures 
= about 14 days old. A uniform suspension was obtained by put- 
ting the spores from the culture in 5 ec. of sterile doubly distilled 
water. A microscopical examination by means of a hanging 
drop was made of a loopful of this suspension. An average of 
6 spores to a field under low power was taken as the standard. 
1 This formula was furnished by Prof. Barre, of Clemson College, S. C. The 
original citation is unknown to the author. 
